Abstract
Guanine-rich DNA sequences commonly form helical quadruplex structures via Hoogsteen hydrogen bonds. The aggregation behavior of the nanoparticles, which are functionalized with four-guanine-terminated 27-base sequences at a nanoparticle-to-DNA ratio of 1:60, is investigated. To some extent, the guanine-quadruplex structures between the gold nanoparticles (GNPs) promote nanoparticle aggregation. However, the coordination site of the metal ion on the nanoparticle surface is partially passivated: the stability of guanine-rich DNA-GNPs is slightly lower than that of the usual DNA-GNPs, and the metal-ion specificity of nanoparticle assembly is substantially decreased. Thus, a mechanism for the aggregation of guanine-rich sequence-modified GNPs is proposed. It is possible to obtain a stable guanine-rich sequence-functionalized nanoparticle solution at high ionic strength by regulating guanine-rich DNA sequences. The controllability of guanine-rich sequence-modified nanoparticles makes the secondary structure of DNA a potentially useful candidate for DNA analysis and disease diagnostics.
Proposed mechanism for the aggregation of G-rich sequence-functionalized GNP
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grants No.20435010, 20375012 and 20205005 ) and the Science Commission of Hunan Province.
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Wu, ZS., Guo, MM., Shen, GL. et al. G-rich oligonucleotide-functionalized gold nanoparticle aggregation. Anal Bioanal Chem 387, 2623–2626 (2007). https://doi.org/10.1007/s00216-007-1126-1
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DOI: https://doi.org/10.1007/s00216-007-1126-1