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Electrochemistry and biosensing activity of cytochrome c immobilized on a mesoporous interface assembled from carbon nanospheres

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Abstract

We report on an amperometric biosensor for hydrogen peroxide. It is obtained via layer-by-layer assembly of ordered mesoporous carbon nanospheres and poly(diallyldimethylammonium) on the surface of an indium tin oxide (ITO) glass electrode and subsequent adsorption of cytochrome c. UV–vis absorption spectroscopy was applied to characterize the process of forming the assembled layers. Cyclic voltammetry revealed a direct and quasi-reversible electron transfer between cytochrome c and the surface of the modified ITO electrode. The surface-controlled electron transfer has an apparent heterogeneous electron-transfer rate constant (k s ) of 5.9 ± 0.2 s−1 in case of the 5-layer electrode. The biosensor displays good electrocatalytic response to the reduction of H2O2, and the amperometric signal increase steadily with the concentration of H2O2 in the range from 5 μM to 1.5 mM. The detection limit is 1 μM at pH 7.4. The apparent Michaelis-Menten constant (K m ) of the sensor is 0.53 mM. We assume that the observation of a direct electron transfer of cytochrome c on mesoporous carbon nanospheres may form the basis for a feasible approach for durable and reliable detection of H2O2.

An amperometric biosensor for hydrogen peroxide has been fabricated via layer-by-layer assembly of mesoporous carbon nanospheres and polyelectrolyte on ITO electrode surface for the adsorption of cytochrome c. The direct electrochemistry and electrocatalytic activity of cytochrome c was achieved on the multilayer-assembled electrode, indicating a good affinity and biocompatibility of mesoporous carbon nanospheres for cytochrome c.

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Acknowledgments

This work was supported by NSFC (20925517, 21175028) and SKLEAC201101. Y. Wang and X. Bian contributed equally to this work.

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Authors and Affiliations

  1. Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, People’s Republic of China

    Ying Wang, Xiaojun Bian, Lei Liao, Jie Zhu, Kai Guo, Jilie Kong & Baohong Liu

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  1. Ying Wang
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  2. Xiaojun Bian
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  3. Lei Liao
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  4. Jie Zhu
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  5. Kai Guo
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  7. Baohong Liu
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Correspondence to Baohong Liu.

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Wang, Y., Bian, X., Liao, L. et al. Electrochemistry and biosensing activity of cytochrome c immobilized on a mesoporous interface assembled from carbon nanospheres. Microchim Acta 178, 277–283 (2012). https://doi.org/10.1007/s00604-012-0834-1

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  • DOI: https://doi.org/10.1007/s00604-012-0834-1

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