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Amperometric sensing of NADH and ethanol using a hybrid film electrode modified with electrochemically fabricated zirconia nanotubes and poly (acid fuchsin)

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Abstract

We report on a glassy carbon electrode (GCE) modified with a film of chitosin containing acid fuchsin (AF) adsorbed onto zirconia nanotubes. The mixture was polymerized by cyclic voltammetric scannings in the potential range from - 0.8 V to +1.3 V in buffer solution to produce a hybrid film electrode (nano-ZrO2/PAF/GCE). The morphology of the hybrid film electrode surface was characterized by scanning electron microscopy. Its electrochemical properties were studied via electrochemical impedance spectroscopy. The electrochemical response of nicotinamide adenine dinucleotide (NADH) was investigated by differential pulse voltammetry and amperometry. The results indicated that the nano-ZrO2/PAF/GCE possesses well synergistic catalytic activity towards NADH. Compared to an unmodified GCE, the oxidation overpotential is negatively shifted by 224 mV, and the oxidation current is significantly increased. Under optimal conditions, the amperometric response is linearly proportional to the concentration of NADH in the 1.0 – 100.0 μM concentration range. Ethanol also can be determined by amperometry if alcohol dehydrogenase and NADH are added to the sample. Two linear relationships between current and alcohol concentration were obtained. They cover the range from 0.03 to 1.0 mM, and from 1.0 to 12.0 mM.

Figure A ZrO2 nanotubes/poly(acid fuchsin) hybrid film modified glassy carbon electrode was electrochemically fabricated. The oxidation overpotential of NADH at the developed nano-ZrO2/PAF/GCE was negatively shifed and the oxidation current was significantly increased. The nano-ZrO2/PAF/GCE was successfully applied to determine NADH and ethanol.

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Acknowledgments

The authors gratefully acknowledge the financial supports from Wuhan Science and Technology Bureau (No. 201160723224), South–Central University for Nationalities (No. XTZ09005) and the Special Fund for Basic Scientific Research of Central Colleges, South−Central University for Nationalities (No. ZZZ10002) and Research foundation of State General Administration of The People’s Republic of China for Quality Supervision and Inspection and Quarantine (No. 2011IK217).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South − Central University for Nationalities, Wuhan, 430074, China

    Xiaoqin Liu, Buhai Li, Guoqing Zhan, Changxian Liu & Chunya Li

  2. Ningbo Entry-exit Inspection and Quarantine Bureau of P. R. C., Ningbo, 315012, China

    Ming Ma

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  1. Xiaoqin Liu
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Correspondence to Chunya Li.

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Liu, X., Li, B., Ma, M. et al. Amperometric sensing of NADH and ethanol using a hybrid film electrode modified with electrochemically fabricated zirconia nanotubes and poly (acid fuchsin). Microchim Acta 176, 123–129 (2012). https://doi.org/10.1007/s00604-011-0701-5

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