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Electrocatalytic detection of NADH and ethanol at glassy carbon electrode modified with electropolymerized films from methylene green

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Abstract

The oxidation of NADH on electropolymerizing methylene green (MG)-modified glassy carbon electrode (GCE) is described. The modified electrode shows an excellent electrocatalytic activity toward NADH oxidation, reducing its overpotential by about 650 mV and exhibits a wide linear range of 5.6–420 μM NADH with the detection limit of 3.8 μM. The electrode displays a good reproducibility and stability and the coexisting species does not affect the determination of NADH. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this electrode. MG-modified GCE can not only be used to detect NADH in biochemical reaction, but also can be used as the potential matrix of the construction of dehydrogenases biosensor.

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Acknowledgements

We gratefully acknowledge the financial support of the National Natural Science Foundation of China (nos. 20505010 and 20473038).

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Authors and Affiliations

  1. Department of Chemistry, Nanjing Normal University, Nanjing, 210097, China

    Z.-H. Dai, F.-X. Liu & J.-C. Bao

  2. Department of Chemistry, Nanjing XiaoZhuang College, Nanjing, 210017, China

    G.-F. Lu

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  1. Z.-H. Dai
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  2. F.-X. Liu
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  3. G.-F. Lu
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  4. J.-C. Bao
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Correspondence to Z.-H. Dai.

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Dai, ZH., Liu, FX., Lu, GF. et al. Electrocatalytic detection of NADH and ethanol at glassy carbon electrode modified with electropolymerized films from methylene green. J Solid State Electrochem 12, 175–180 (2008). https://doi.org/10.1007/s10008-007-0378-1

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  • DOI: https://doi.org/10.1007/s10008-007-0378-1

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