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Electrocatalytic oxidation of the reduced nicotinamide adenine dinucleotide at carbon ionic liquid electrode modified with polythionine/multi-walled carbon nanotubes composite

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Abstract

A carbon ionic liquid electrode (CILE) was modified with a polythionine (PTh)/multi-walled carbon nanotubes (MWCNTs) composite and used for the detection of reduced nicotinamide adenine dinucleotide (NADH). The electrode was prepared by electrochemical polymerization of thionine on the MWCNTs in neutral medium. Cyclic voltammetry indicated that the electrode was capable of mediating the oxidation of NADH at an overpotential as low as 0.03 V. Amperometric experiments showed that a sensitive and stable response towards NADH is obtained within 5 s. The linear range for the determination of NADH is from 0.8 μmol L−1 to 422 μmol L−1, with a detection limit of 0.26 μmol L−1 (S/N = 3). The wide linear range, lower detection limit and faster response towards NADH suggests that the new method potentially is useful for developing NAD+-dependent enzyme-based biosensors.

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Acknowledgement

This work was financially supported by the National Outstanding Youth Foundations of China, National Science Foundation of China (50725825) and Special Research Found for the Doctoral Program of Higher Education of China (20060532006).

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Authors and Affiliations

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Hunan, Changsha, 410082, People’s Republic of China

    Nannan Mai, Xiaoying Liu, Xiandong Zeng, Liang Xing, Wanzhi Wei & Shenglian Luo

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  1. Nannan Mai
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  2. Xiaoying Liu
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  3. Xiandong Zeng
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  4. Liang Xing
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  5. Wanzhi Wei
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  6. Shenglian Luo
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Correspondence to Wanzhi Wei or Shenglian Luo.

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Mai, N., Liu, X., Zeng, X. et al. Electrocatalytic oxidation of the reduced nicotinamide adenine dinucleotide at carbon ionic liquid electrode modified with polythionine/multi-walled carbon nanotubes composite. Microchim Acta 168, 215–220 (2010). https://doi.org/10.1007/s00604-009-0285-5

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  • DOI: https://doi.org/10.1007/s00604-009-0285-5

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