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Solid phase extraction with electrospun nanofibers for determination of retinol and α-tocopherol in plasma

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Abstract

A novel packed-fiber solid phase extraction procedure based on electrospun nanofibers for simultaneous determination of vitamins A (retinol) and E (α-tocopherol) in human plasma has been developed. Parameters affecting extraction efficiency were investigated in detail. The limit of detection is 0.01 μg mL−1 for retinol, and 0.3 μg mL−1 for α-tocopherol. The linear range is from 0.05 to 2.0 μg mL−1 for retinol, and from 0.5 to 30 μg mL−1 for α-tocopherol. The precision (RSD) is <6%, and the relative recovery >90%. The method was applied to analysis of retinol and α-tocopherol in human plasma with satisfactory results.

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Acknowledgement

This work was jointly supported by Jiangsu Science and Technology Department (Grant No. BG2007044), Suzhou Science and Technology Department (Grant No. SYJG0912), and Jiangsu Preventive Medicine Foundation (Grant No. Y2006025).

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Authors and Affiliations

  1. Key Laboratory of Child Development and Learning Science, Ministry of Education, Research Center For Learning Science, Southeast University, Si Pai Lou 2, Nanjing, 210096, People’s Republic of China

    Xuejun Kang

  2. Lab of Environment and Biosafety, Research Institute of Southeast University in Suzhou, School of Public Health, Dushu Lake Higher Education Town, Suzhou, 215103, People’s Republic of China

    Zhiyong Liu, Xuejun Kang & Fang Fang

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  1. Zhiyong Liu
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  2. Xuejun Kang
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  3. Fang Fang
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Correspondence to Xuejun Kang.

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Liu, Z., Kang, X. & Fang, F. Solid phase extraction with electrospun nanofibers for determination of retinol and α-tocopherol in plasma. Microchim Acta 168, 59–64 (2010). https://doi.org/10.1007/s00604-009-0263-y

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  • DOI: https://doi.org/10.1007/s00604-009-0263-y

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