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Electrochemistry and biosensing of glucose oxidase immobilized on Pt-dispersed mesoporous carbon

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Abstract

A method for immobilizing proteins in a carbon mesoporous material (CMM) containing platinum nanoparticles (Pt-NPs) is demonstrated. Compared to pure CMM or carbon nanotubes, CMM containing Pt-NPs enhances the electron transfer and redox properties of redox enzymes, such as glucose oxidase (GOx), due to a cooperative effect of Pt-NPs and CMM. The quasi-reversible electron transfer of GOx in this system is probed, and the apparent heterogeneous electron transfer rate constants are found to be 66% larger than in pure CMM. The GOx/Pt-CMM based glucose biosensor enables the determination of glucose at a potential of 600 mV (vs. SCE). Its detection limit is 10 times lower, and the sensitivity is 16 times higher than that of the respective biosensor without Pt-NPs.

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Acknowledgements

This study was supported by the NNSF of China (20775016), Shanghai Leading Academic Discipline Project B108, B109, STCSM 09JC1402600 and Shuguang Project 06SG02.

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Authors and Affiliations

  1. Department of Chemistry, Shanghai Key Lab of Molecular Catalysis and Novel Materials, and Institutes of Advanced Materials and Biomedical Sciences, Fudan University, Shanghai, 200433, China

    Chunping You, Xiang Li, Song Zhang, Jilie Kong, Dongyuan Zhao & Baohong Liu

  2. Technology Center, Bright Dairy and Food Co Ltd, Shanghai, 200436, China

    Chunping You

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  1. Chunping You
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  2. Xiang Li
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  3. Song Zhang
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  4. Jilie Kong
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  5. Dongyuan Zhao
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  6. Baohong Liu
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Correspondence to Baohong Liu.

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You, C., Li, X., Zhang, S. et al. Electrochemistry and biosensing of glucose oxidase immobilized on Pt-dispersed mesoporous carbon. Microchim Acta 167, 109 (2009). https://doi.org/10.1007/s00604-009-0228-1

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  • DOI: https://doi.org/10.1007/s00604-009-0228-1

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