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Ectomycorrhizal synthesis between two Tuber species and six tree species: are different host-fungus combinations having dissimilar impacts on host plant growth?

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Abstract

Truffle cultivation has drawn more and more attention for its high economic and ecological values in the world. To select symbionts suitable for cultivation purposes, we conducted greenhouse-based mycorrhization trials of two Tuber species (T. formosanum and T. pseudohimalayense) with five broad-leaved tree species (Corylus yunnanensis, Quercus aliena var. acutiserrata, Q. acutissima, Q. robur, Q. variabilis) and one conifer species (Pinus armandii). Axenically germinated seedlings of all tree species were either inoculated, or not, with spore suspensions of these two truffles in the greenhouse. Eight months after inoculation, T. formosanum or T. pseudohimalayense ectomycorrhizae were successfully formed on these six tree species, as evidenced by both morphological and molecular analyses. All selected trees showed good receptivity to mycorrhization by both fungi, with average colonization rates visually estimated at 40–50%. Plant growth, photosynthesis, and nutrient uptake were assessed 2 years after inoculation and were mainly affected by host species. Mycorrhization by both fungi significantly improved P uptake of the hosts, and the interaction between truffle species and host plant species had significant effects on leaf water and leaf K concentrations. In addition, a significantly negative correlation between leaf Ca and leaf C concentration was found across all the seedlings. In addition, mycorrhization had slightly increased plant stem and canopy, but had no significant effects on plant photosynthesis. Overall, these results suggest that the effects of these two Tuber ECMF on plant growth and nutrient acquisition depend on the identity of the host species. Moreover, all selected plant species could be symbiotic partners with either T. pseudohimalayense or T. formosanum for field cultivation purposes.

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Acknowledgements

The authors are grateful to Xinhua He for reviewing the manuscript and providing useful suggestions.

Funding

This work was supported by projects from the National Key Research and Development Program of China (2021YFD1600404), Yunnan Key Project of Science and Technology (202102AE090034), and Yunnan High Level Talent Plan to Xinhua He and Yanliang Wang.

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  1. Lan-Lan Huang and Yan-Liang Wang are co-first authors.

Authors and Affiliations

  1. College of Resources and Environment, Yunnan Agricultural University, Kunming, 650201, China

    Lan-Lan Huang & Yong-Mei Li

  2. The Germplasm Bank of Wild Species, Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Science, 132 Lanhei Road, Yunnan, Kunming, 650201, China

    Lan-Lan Huang, Yan-Liang Wang, Ran Wang, Peng Zhang & Fu-Qiang Yu

  3. Mycotree C/-Southern Woods Nursery, 1002 Robinsons Road, RD8, Christchurch, New Zealand

    Alexis Guerin-Laguette

  4. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China

    Alexis Guerin-Laguette

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  1. Lan-Lan Huang
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Huang, LL., Wang, YL., Guerin-Laguette, A. et al. Ectomycorrhizal synthesis between two Tuber species and six tree species: are different host-fungus combinations having dissimilar impacts on host plant growth?. Mycorrhiza 32, 341–351 (2022). https://doi.org/10.1007/s00572-022-01081-6

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