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First detection of Endogone ectomycorrhizas in natural oak forests

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Abstract

The order Endogonales in Mucoromycotina, an early divergent lineage of fungi, includes ectomycorrhizal (EM) fungi. This order is therefore considered a key taxon for elucidation of the evolution of EM associations. Recent studies have revealed high diversity of EM lineages of Basidiomycota and Ascomycota; however, EM associations of Endogonales and its relatives remain largely unknown. In this study, EM root tips with a unique fungal sheath, with aseptate and highly branched hyphae of variable widths, were identified in Quercus acutissima and Quercus crispula forests in the temperate zone of Japan. The mycobionts were confirmed as Endogone sp., which were placed as a sister clade of Endogone pisiformis, based on phylogenetic analyses of the small and large subunits of the nuclear ribosomal RNA and elongation factor-1α genes. This is the first report of EM of Endogone in natural forests of the Northern Hemisphere and the first finding on Quercus.

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Acknowledgments

We are grateful to the members of the Laboratory of Mycology, Sugadaira Montane Research Center, University of Tsukuba, for support for our sampling. We would like to thank Dr. T. Y. James for allowing the use of sequence data in the AFTOL database. We also thank the technical staff in the Division of Instrumental Analysis, Research Center for Human and Environmental Sciences, Shinshu University for DNA sequencing. This study was supported in part by the Research Fellowships for Young Scientists (No. JP14J09199) and KAKENHI (No. JP25291084 and JP15H01751) from the Japan Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304, Minami-minowa, Nagano, 399-4598, Japan

    Kohei Yamamoto & Akiyoshi Yamada

  2. Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University, 4-101, Koyama, Tottori, 680-8553, Japan

    Naoki Endo

  3. Sugadaira Montane Research Center, University of Tsukuba, 1278-294, Sugadaira, Ueda, Nagano, 386-2204, Japan

    Yousuke Degawa

  4. Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304, Minami-minowa, Nagano, 399-4598, Japan

    Masaki Fukuda & Akiyoshi Yamada

  5. Division of Terrestrial Ecosystem, Institute of Mountain Science, Shinshu University, 8304, Minami-minowa, Nagano, 399-4598, Japan

    Akiyoshi Yamada

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  1. Kohei Yamamoto
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Correspondence to Kohei Yamamoto.

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Yamamoto, K., Endo, N., Degawa, Y. et al. First detection of Endogone ectomycorrhizas in natural oak forests. Mycorrhiza 27, 295–301 (2017). https://doi.org/10.1007/s00572-016-0740-1

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