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Arbuscular mycorrhizal symbiosis modulates antioxidant response in salt-stressed Trigonella foenum-graecum plants

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Abstract

An experiment was conducted to evaluate the influence of Glomus intraradices colonization on the activity of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), peroxidase (PX), ascorbate peroxidase (APX), and glutathione reductase (GR)] and the accumulation of nonenzymatic antioxidants (ascorbic acid, α-tocopherol, glutathione, and carotenoids) in roots and leaves of fenugreek plants subjected to varying degrees of salinity (0, 50, 100, and 200 mM NaCl) at two time intervals (1 and 14 days after saline treatment, DAT). The antioxidative capacity was correlated with oxidative damage in the same tissue. Under salt stress, lipid peroxidation and H2O2 concentration increased with increasing severity and duration of salt stress (DoS). However, the extent of oxidative damage in mycorrhizal plants was less compared to nonmycorrhizal plants. The study reveals that mycorrhiza-mediated attenuation of oxidative stress in fenugreek plants is due to enhanced activity of antioxidant enzymes and higher concentrations of antioxidant molecules. However, the significant effect of G. intraradices colonization on individual antioxidant molecules and enzymes varied with plant tissue, salinity level, and DoS. The significant effect of G. intraradices colonization on antioxidative enzymes was more evident at 1DAT in both leaves and roots, while the concentrations of antioxidant molecules were significantly influenced at 14DAT. It is proposed that AM symbiosis can improve antioxidative defense systems of plants through higher SOD activity in M plants, facilitating rapid dismutation of O2 - to H2O2, and subsequent prevention of H2O2 build-up by higher activities of CAT, APX, and PX. The potential of G. intraradices to ameliorate oxidative stress generated in fenugreek plants by salinity was more evident at higher intensities of salt stress.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

DAT:

Days after treatment

DoS:

Duration of stress

GR:

Glutathione reductase

M:

Mycorrhizal

NM:

Nonmycorrhizal

PX:

Peroxidase

SOD:

Superoxide dismutase

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Acknowledgments

Heikham Evelin is grateful to the Council of Scientific and Industrial Research, New Delhi, India, and the University of Delhi for financial assistance.

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Authors and Affiliations

  1. Applied Mycology Laboratory, Department of Botany, University of Delhi, Delhi, 110 007, India

    Heikham Evelin & Rupam Kapoor

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  1. Heikham Evelin
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  2. Rupam Kapoor
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Correspondence to Rupam Kapoor.

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Evelin, H., Kapoor, R. Arbuscular mycorrhizal symbiosis modulates antioxidant response in salt-stressed Trigonella foenum-graecum plants. Mycorrhiza 24, 197–208 (2014). https://doi.org/10.1007/s00572-013-0529-4

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