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Highly-efficient SERS detection for E. coli using a microfluidic chip with integrated NaYF4:Yb,Er@SiO2@Au under near-infrared laser excitation

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Abstract

A reliable NaYF4:Yb,Er material assembled from Au (UC@SiO2@Au), as the substrate, was firstly integrated into a microfluidic chip and employed to detect R6G and E. coli by surface enhanced Raman spectroscopy (SERS) under a near-infrared excitation. In this microfluidic SERS chip, NaYF4:Yb,Er can convert near-infrared (NIR) light into visible light to promote localized surface plasmon resonance (LSPR) of gold nanoparticles to enhance the SERS signal. Moreover, a microfluidic chip could provide a good survival environment of bacteria, and NIR laser had a relatively smaller damage to the E. coli to improve stability of SERS signals of bacteria. According to synthesis conditions, the SERS substrate was fabricated and evaluated in a microfluidic chip. It shows uniformity with RSD of 11.5% for the Raman vibrations at 926 cm−1, and sensitivity with detection limits of 10−9 M for R6G under a 785 nm excitation. And it also exhibited an excellent sensitivity and stability for E. coli SERS detection under the near-infrared laser excitation. The advantages of NIR excitation in our SERS sensing expands the application range of the microfluidic SERS chip, and also promise a new research direction for the synthesis and applications of SERS-active nanostructures.

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Acknowledgements

The authors acknowledge the financial support Supported by the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant no. KJQN202001122); the Start up Project of Scientific Research Funds for the Chongqing Universities of Technology (2017ZD41 and 2017ZD06) and the Chongqing Research Program of Basic Research and Frontier Technology Fund (cstc2017jcyjAX0462).

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Authors and Affiliations

  1. College of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China

    Chunyan Wang, Xiaoqin Mu & Kunyuan Zhang

  2. Chongqing DeWen ZhiShang Education Technology Co. LTD, Chongqing, 400042, China

    Jia Huo

  3. College of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing, 400041, China

    Bei Zhang

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  1. Chunyan Wang
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  2. Xiaoqin Mu
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  3. Jia Huo
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  4. Bei Zhang
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  5. Kunyuan Zhang
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Correspondence to Chunyan Wang.

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Wang, C., Mu, X., Huo, J. et al. Highly-efficient SERS detection for E. coli using a microfluidic chip with integrated NaYF4:Yb,Er@SiO2@Au under near-infrared laser excitation. Microsyst Technol 27, 3285–3291 (2021). https://doi.org/10.1007/s00542-020-05087-y

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  • DOI: https://doi.org/10.1007/s00542-020-05087-y

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