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Cardioprotective effect and mechanism of action of landiolol on the ischemic reperfused heart

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Abstract

Purpose

The authors examined the cardioprotective effect of landiolol, an ultra short-acting, highly selective β1-blocker, and its role in cardiac work, antioxidative effect, and sarcoplasmic reticulum (SR) function in hearts subjected to ischemia-reperfusion.

Methods

Isolated guinea pig hearts were subjected to ischemia-reperfusion by stopping the perfusion for 45 min and reperfusing. Before the ischemia, hearts were treated with landiolol (20, 100, or 500 µM) for 15 min (LAN group). In another set of experiments, before ischemia, hearts were washed out for 15 min after treatment with landiolol (WO group). In other hearts, the tissue concentration of malondialdehyde was measured after reperfusion. We also examined the phosphorylation of phospholamban at Ser16 and Thr17residues to evaluate the SR function.

Results

After 90 min of reperfusion, left ventricular pressure (LVP) was restored significantly in the LAN-500 µM group regardless of heart rate. However, the improvement in recovery in LVP disappeared in the WO group. The tissue malondialdehyde levels were decreased in the LAN group compared with those in the control group. In the control group, the phosphorylation of phospholamban at Ser16 and Thr17 residues was markedly increased after reperfusion. Landiolol at 500 µM suppressed the increase of phosphorylation at Ser16 residues.

Conclusion

The present study demonstrated that landiolol had a lipid peroxidation-reducing effect and suppressed the increase in phospholamban phosphorylation at the Ser16 residue in hearts subjected to ischemia-reperfusion. These findings indicate that landiolol may have an anti-ischemic effect, via an antioxidant effect and/or via preserving SR function during the ischemic period.

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Authors and Affiliations

  1. Department of Anesthesiology, Sapporo Medical University, School of Medicine, S-1, W-16, Chuo-ku, Sapporo, 060-8543, Japan

    Saori Kimura-Kurosawa, Noriaki Kanaya, Noriko Kamada, Naoyuki Hirata, Masayasu Nakayama & Akiyoshi Namiki

Authors
  1. Saori Kimura-Kurosawa
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  2. Noriaki Kanaya
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  3. Noriko Kamada
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  4. Naoyuki Hirata
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  5. Masayasu Nakayama
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  6. Akiyoshi Namiki
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Additional information

Presented in part at the annual meeting of the American Society of Anesthesiologists, Las Vegas, Nevada, October 23–27, 2004.

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Kimura-Kurosawa, S., Kanaya, N., Kamada, N. et al. Cardioprotective effect and mechanism of action of landiolol on the ischemic reperfused heart. J Anesth 21, 480–489 (2007). https://doi.org/10.1007/s00540-007-0558-2

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  • DOI: https://doi.org/10.1007/s00540-007-0558-2

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