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Comparative cytological and transcriptomic analysis of pollen development in autotetraploid and diploid rice

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Abstract

Autotetraploid rice has greater genetic variation and higher vigor than diploid rice, but low pollen fertility is one of the major reasons for low yield of autotetraploid rice. Very little is known about the molecular mechanisms of low pollen fertility of autotetraploid rice. In this study, cytological observations and microarray analysis were used to assess the genetic variation during pollen development in autotetraploid and diploid rice. Many abnormal chromosome behaviors, such as mutivalents, lagged chromosomes, asynchronous cell division, and so on, were found during meiosis in autotetraploid. Microsporogenesis and microgametogenesis in autotetraploid rice was similar to diploid rice, but many different kinds of abnormalities, including microspores degeneration, multi-aperture, and abnormal cell walls, were found in autotetraploid rice. Compared with diploid rice, a total of 1,251 genes were differentially expressed in autotetraploid rice in pollen transcriptome, among them 1,011 and 240 genes were up-regulated and down-regulated, respectively. 124 and 6 genes were co-up-regulated and co-down-regulated during three pollen development stages, respectively. These results suggest that polyploidy induced up-regulation for most of the genes during pollen development. Quantitative RT-PCR was done to validate 12 differentially expressed genes selected from functional categories based on the gene ontology analysis. These stably expressed genes not only related to the pollen development genes, but also involved in cell metabolism, cell physiology, binding, catalytic activity, molecular transducer activity, and transcription regulator activity. The present study suggests that differential expression of some key genes may lead to complex gene regulation and abnormal pollen development in autotetraploid rice.

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Acknowledgments

The authors appreciated the supports from Prof. Guiquan Zhang for donating Taichung-65. We also thank Mr. MO Dunzhou, Ms. ZHAO Xingjuan, and YU Shuhong for technical assistance. This work was supported by the NSFC (31270352 and 31210103023 to X. D. Liu), Guangdong provincial key platform of University and major research project (Natural Science)—characteristic innovation project [Yue-JK2014 (65) to X. D. Liu], and the Guangdong Provincial Science and Technique Special Fund for State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (2012).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, China

    Jinwen Wu, Muhammad Qasim Shahid, Haibin Guo, Wei Yin, Zhixiong Chen, Lan Wang, Xiangdong Liu & Yonggen Lu

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  1. Jinwen Wu
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  2. Muhammad Qasim Shahid
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  3. Haibin Guo
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  4. Wei Yin
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  5. Zhixiong Chen
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  6. Lan Wang
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  7. Xiangdong Liu
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  8. Yonggen Lu
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Corresponding authors

Correspondence to Xiangdong Liu or Yonggen Lu.

Additional information

Communicated by David Twell.

J. Wu and M. Q. Shahid have contributed equally to this work.

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Wu, J., Shahid, M.Q., Guo, H. et al. Comparative cytological and transcriptomic analysis of pollen development in autotetraploid and diploid rice. Plant Reprod 27, 181–196 (2014). https://doi.org/10.1007/s00497-014-0250-2

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  • DOI: https://doi.org/10.1007/s00497-014-0250-2

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