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Abnormal PMC microtubule distribution pattern and chromosome behavior resulted in low pollen fertility of an intersubspecific autotetraploid rice hybrid

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Abstract

Intersubspecific autotetraploid rice hybrids have high heterosis in both vegetative and reproductive growth, but low seed set hinders commercial utilization of autotetraploid rice. Autotetraploid rice hybrids with high and low pollen fertility were used in the present study to compare microtubule distribution patterns and chromosome behavior during pollen mother cell (PMC) meiosis, using indirect immunofluorescence laser scanning confocal microscopy. Microtubule distribution patterns of autotetraploid hybrids were similar to diploid rice, but many different kinds of abnormalities were found in the hybrid with low pollen fertility and seed set. Abnormal microtubule organization including structurally distorted microtubules at pachytene, loosely knitted perinuclear microtubules at diakinesis during prophase I, and abnormal spindles, viz. multipoles, loss of spindle pole focus, abnormal size of spindles, and so on, were found at metaphase I and metaphase II. Some cells developed into triad with no formation of tetrad. Abnormal chromosome behaviors included high percentage of multivalents, chromosome lagging, chromosome bridges, and micronuclei. All these abnormalities were found more frequently in low-fertile hybrid than in high-fertile hybrid. These results suggest that abnormal microtubule distribution pattern is an important factor which affects pollen fertility and percentage seed set in autotetraploid rice, and may also have a close relationship with chromosome behavior.

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Acknowledgments

The authors thank Dr. Li Jin-Quan, Fu Xue-Lin, and Assc. Prof. Yang Bing-Yao, Su Wei, Ms Yu Shu-Hong, and Zhao Xing-Juan for their help in the laboratory and field. This research was supported by the National Science Foundation of China (30771328) and Joint Fund of the National Natural Science Foundation of China, Guangdong Province (Grant No. U0631003).

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  1. Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, Guangdong, China

    Jin-Hua He, Muhammad Qasim Shahid, Zhi-Xiong Chen, Xing-An Chen, Xiang-Dong Liu & Yong-Gen Lu

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  1. Jin-Hua He
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  2. Muhammad Qasim Shahid
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  3. Zhi-Xiong Chen
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  4. Xing-An Chen
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  5. Xiang-Dong Liu
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Correspondence to Xiang-Dong Liu or Yong-Gen Lu.

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He, JH., Shahid, M.Q., Chen, ZX. et al. Abnormal PMC microtubule distribution pattern and chromosome behavior resulted in low pollen fertility of an intersubspecific autotetraploid rice hybrid. Plant Syst Evol 291, 257–265 (2011). https://doi.org/10.1007/s00606-010-0386-y

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