Abstract
Variation is the phenomenon where individuals of a population differ from each other. The variation or diversity can have morphological manifestation or genetic basis. Individual identification based on morphological record is the most common practice in Jatropha. Therefore, in order to find a proper method for estimation of genetic diversity and genetic relationships among different germplasm of Jatropha curcas L., random amplified polymorphic DNA (RAPD) technique based on polymerase chain reaction (PCR) was used for described purpose. Out of 55 decamer primers tested, 26 primers produced good amplification products. A total of 6,011 amplification products were scored from which only 1,859 bands (30.92%) were found to be polymorphic and the size of bands ranged from 300 to 2,500 bp. Unweighted pair group method using arithmetic average cluster analysis revealed clear genetic difference among J. curcas germplasm. The scientific data presented in this study suggests that RAPD-PCR could be used as a valuable tool for estimation of genetic diversity and genetic relationship among germplasm of J. curcas L.
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Abdalla AM, Reddy OUK, El-Zik KM, Pepper AE (2000) Genetic diversity and relationships of diploid and tetraploid cottons revealed using AFLP. Theor Appl Genet 102:222–229. doi:10.1007/s001220051639
Bradshaw HD, Wilbert SM, Otto KG, Schemske DW (1995) Genetic mapping of floral traits associated with reproductive isolation in monkey flowers (Mimulus). Nature 376:762–765. doi:10.1038/376762a0
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA preparation, version II. Plant Mol Biol Rep 1:19–21. doi:10.1007/BF02712670
Graner A, Ludwig WF, Melchinger AE (1994) Relationships among European barley germplasm. Crop Sci 34:1199–1205
Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270
Kalendar R, Grob T, Regina M, Suoniemi A, Schulman A (1999) IRAP and REMAP, two new retrotransposon based DNA fingerprinting techniques. Theor Appl Genet 98:704–711. doi:10.1007/s001220051124
Kumar RV, Ahlawat SP, Gupta VK, Handa AK, Yadav VP, Tripathi YK (2005) Jatropha-Jaiv Indhan ka Shrotra, Technical Bulletin, NRCAF, Jhansi, pp 1–12
Kumar RV, Tripathi YK, Izhaki I, Yadav VP, Ahlawat SP (2008) Intraspecific variation and interrelationships between morphology, nutritional content and enzymatic activity of Jatropha curcas L. Curr Sci 95(2):239–243
Ma R, Mattila TY, Pulli S (2004) Phylogenetic relationships among genotypes of worldwide collection of spring and winter ryes (Secale cereale L.) determined by RAPD-PCR markers. Hereditas 140:210–221. doi:10.1111/j.1601-5223.2004.01844.x
Pejic I, Ajmone-Marsan P, Morgante M, Kozumplick V, Castiglioni P, Taramino G, Motto M (1998) Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs and AFLPs. Theor Appl Genet 97:1248–1255. doi:10.1007/s001220051017
Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta PK (2000) The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theor Appl Genet 100:584–592
Ramsay L, Macaulay M, Cardle R, Morgante M, Geglivanissevich S, Maestri E, Powell W, Waugh R (1999) Intimate association of microsatellite repeats with retrotransposons and other dispersed repetitive elements in barley. Plant J 17:415–425. doi:10.1046/j.1365-313X.1999.00392.x
Ribaut JM, Hoisington D (1998) Marker-assisted selection, new tools and strategies. Trends Plant Sci 3:236–239. doi:10.1016/S1360-1385(98)01240-0
Rohlf FJ (1998) NTSYSpc numerical taxonomy and multivariate analysis system, version 2.2. Exeter Software, Setauket
Russel JR, Fuller JD, Macauley M, Hatz BG, Jagoor A, Powel W, Waugh R (1997) Direct comparison of level of genetic variation among barley accession detected by RFLPs, AFLPs, SSRs and RAPDs. Theor Appl Genet 95:714–722. doi:10.1007/s001220050617
Russel JR, Ellis RP, Thomas WTB, Waugh R, Provan J, Booth A, Fuller J, Lawrence P, Young G, Powell W (2000) A retrospective analysis of spring barley germplasm development from foundation genotypes to currently successful cultivars. Mol Breed 6:553–568. doi:10.1023/A:1011372312962
Schut JW, Qi X, Stam P (1997) Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barley. Theor Appl Genet 95:1161–1168. doi:10.1007/s001220050677
Sujatha M, Prabakaran AJ (1997) Characterization and utilization of Indian Jatropha. Indian J Plant Genet Resour 10(1):123–128
Tripathi YK, Gurha P, Ghosh D, Kumar RV, Goswami HK, Prakash V (2007) Determination of phylogenetic relationship among Isoetes species using random primers. Turk J Bot 31:1–6
Williams JGK, Kubelik AR, Livak KJ (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
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The authors are very much thankful to Dr. S. K. Dhyani, Director, NRCAF, Jhansi for providing facilities to conduct this study.
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Communicated by R. Hampp.
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Kumar, R.V., Tripathi, Y.K., Shukla, P. et al. Genetic diversity and relationships among germplasm of Jatropha curcas L. revealed by RAPDs. Trees 23, 1075–1079 (2009). https://doi.org/10.1007/s00468-009-0350-z
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DOI: https://doi.org/10.1007/s00468-009-0350-z