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Optimising enzyme production by bakers yeast in continuous culture: physiological knowledge useful for process design and control

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Abstract

Saccharomyces cerevisiae was grown in aerobic continuous culture on a defined minimal medium, with glucose (40 g.l−1) as the growth-limiting carbon source, to acquire knowledge useful in process design and for model-based control. Steady-state concentrations of biomass, glucose, ethanol and activities of model products alcohol dehydrogenase, hexokinase, malate dehydrogenase, glucose-6-phosphate dehydrogenase and iso-citrate dehydrogenase were determined at dilution rates (D) between 0.06 h−1 and 0.323 h−1 (close to μmax). Enzyme activities showed productivity trends related to the transition from oxidative to oxido-reductive growth. Conclusions are drawn from the data with regard to designing a new process for production of intracellular enzymes. Issues of process stability as well as productivity are discussed.

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Abbreviations

ADH:

alcohol dehydrogenase

D:

dilution rate

DCW:

dry cell weight

G6PDH:

glucose-6-phosphate dehydrogenase

ICDH:

isocitrate dehydrogenase

HK:

hexokinase

MDH:

malate dehydrogenase

μ:

specific growth rate

q eth :

specific ethanol production rate

RQ:

respiratory quotient

U:

unit of enzyme activity

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Authors and Affiliations

  1. Advanced Centre for Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, U.K.

    M. Bulmer, I. D. L. Bogle & N. Titchener-Hooker

  2. Centre for Process Systems Engineering, Imperial College of Science Technology and Medicine, SW7 2BY, London, U.K.

    M. E. Gregory

Authors
  1. M. Bulmer
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  2. I. D. L. Bogle
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  3. N. Titchener-Hooker
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  4. M. E. Gregory
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Bulmer, M., Bogle, I.D.L., Titchener-Hooker, N. et al. Optimising enzyme production by bakers yeast in continuous culture: physiological knowledge useful for process design and control. Bioprocess Engineering 15, 239–245 (1996). https://doi.org/10.1007/BF02391584

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