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A study of the effect of specific growth rate and acetate on recombinant protein production of Escherichia coli JM107

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Abstract

The growth of Escherichia coli in fed-batch and continuous culture is examined and results show that α-amylase production is strongly dependent on specific growth rate (dilution rate) of the culture and production is greatest at an intermediate rate. Using continuous culture, it has also been found that the presence of acetate above a certain concentration reduces both μmax, and the production of recombinant protein.

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Authors and Affiliations

  1. Advanced Centre for Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, UK

    Claire Turner & Michael K. Turner

  2. Centre for Process Systems Engineering, Imperial College of Science, Technology and Medicine, SW7, London, UK

    Malcolm E. Gregory

Authors
  1. Claire Turner
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  2. Malcolm E. Gregory
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  3. Michael K. Turner
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Turner, C., Gregory, M.E. & Turner, M.K. A study of the effect of specific growth rate and acetate on recombinant protein production of Escherichia coli JM107. Biotechnol Lett 16, 891–896 (1994). https://doi.org/10.1007/BF00128620

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  • DOI: https://doi.org/10.1007/BF00128620

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