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Recycling of cooking oil fume condensate for the production of rhamnolipids by Pseudomonas aeruginosa WB505

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Abstract

Rhamnolipids (RLs) are anionic biosurfactants with great application potential. This study explored the possibility of producing RLs from cooking oil fume condensates (COFCs) collected from range hoods. A mutant of Pseudomonas aeruginosa AB93066 was obtained and used to produce RLs from COFCs as a substrate. RL yields in a 7-L fermenter reached 12.3 g/L, and MALDI–TOF MS showed that Rha2–C10–C10 and Rha–C10–C10 are the most abundant (39.6% and 26.4%, respectively) RL components. The critical micellar concentration (CMC) of the RLs was 45.0 mg/L and the surface tension of water decreased from 60.5 to 25.3 mN/m. Using six kinds of common hydrocarbons as indices, the emulsification coefficients of the RLs obtained were found to exceed 60%; in particular, the emulsification coefficient for benzene was 80.3%. COFCs provide an inexpensive alternative as a substrate for RL production, and the synthetic process is relatively harmless and economical.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 21778022), the Program of Introducing Talents of Discipline to Universities (No. 111-2-06), the Fundamental Research Funds for the Central Universities (No. JUSRP51632A), and the National First-class Discipline Program of Light Industry Technology and Engineering (No. LITE2018-17).

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Authors and Affiliations

  1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education; School of Biotechnology, Jiangnan University, 1800 Lihu Av., Wuxi, 214122, Jiangsu, China

    Jianrong Wu, Jingbo Zhang, Hongtao Zhang, Minjie Gao & Xiaobei Zhan

  2. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Av., Wuxi, 214122, Jiangsu, China

    Liming Liu

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  1. Jianrong Wu
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Wu, J., Zhang, J., Zhang, H. et al. Recycling of cooking oil fume condensate for the production of rhamnolipids by Pseudomonas aeruginosa WB505. Bioprocess Biosyst Eng 42, 777–784 (2019). https://doi.org/10.1007/s00449-019-02081-1

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