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Biodegradation of alkali lignin by a newly isolated Rhodococcus pyridinivorans CCZU-B16

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Abstract

Based on the Prussian blue spectrophotometric method, one high-throughput screening strategy for screening lignin-degrading microorganisms was built on 24-well plate at room temperature. One high activity of alkali lignin-degrading strain Rhodococcus pyridinivorans CCZU-B16 was isolated from soil. After the optimization of biodegradation, 30.2% of alkali lignin (4 g/L) was degraded under the nitrogen-limited condition (30/1 of C/N ratio; g/g) at 30 °C for 72 h. It was found that syringyl (S) units and guaiacyl (G) in lignin decreased after biodegradation. Moreover, the accumulated lipid in cells had a fatty acid profile rich in C16 and C18 with four major constituent fatty acids including palmitic acid (C16:0; 22.4%), palmitoleic acid (C16:1; 21.1%), stearic acid (C18:0; 16.2%), and oleic acid (C18:1; 23.1%). In conclusion, Rhodococcus pyridinivorans CCZU-B16 showed high potential application in future.

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Acknowledgements

This work was financially supported by the Natural Science Foundation of Jiangsu Province (No. BK20141172), the Key Laboratory of Fermentation Engineering (Ministry of Education), and the Green Catalysis and Applied Enzyme Group of Changzhou Vocational Institute of Engineering (No. 111308002216006).

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Authors and Affiliations

  1. Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, People’s Republic of China

    Gang-Gang Chong, Xiao-Jun Huang, Jun-Hua Di, Dao-Zhu Xu, Yu-Cai He & Ya-Nan Pei

  2. Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, People’s Republic of China

    Yu-Cai He & Cui-Luan Ma

  3. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, People’s Republic of China

    Yu-Cai He & Ya-Jie Tang

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  1. Gang-Gang Chong
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Chong, GG., Huang, XJ., Di, JH. et al. Biodegradation of alkali lignin by a newly isolated Rhodococcus pyridinivorans CCZU-B16. Bioprocess Biosyst Eng 41, 501–510 (2018). https://doi.org/10.1007/s00449-017-1884-x

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  • DOI: https://doi.org/10.1007/s00449-017-1884-x

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