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Elucidating the binding efficacy of β-galactosidase on graphene by docking approach and its potential application in galacto-oligosaccharide production

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Abstract

Herein, we propose the synthesis and characterization of graphene for the immobilization of β-galactosidase for improved galacto-oligosaccharide (GOS) production. The size of synthesized graphene was observed to be 25 nm by TEM analysis while interaction of enzyme with the nanosupport was observed by FTIR spectroscopy. Docking was obtained using molecular docking program Dock v.6.5 while the visual analyses and illustration of protein–ligand complex were investigated by utilizing chimera v.1.6.2 and PyMOL v.1.3 softwares. Immobilized β-galactosidase (IβG) showed improved stability against various physical and chemical denaturants. K m of IβG was increased to 6.41 mM as compared to 2.38 mM of soluble enzyme without bringing significant change in V max value. Maximum GOS content also registered an increase in lactose conversion. The maximum GOS production was achieved by immobilized enzyme at specific temperature and time. Hence, the developed nanosupport can be further exploited for developing a biosensor involving β-galactosidase or for immobilization of other industrially/therapeutically important enzymes.

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Abbreviations

GO:

Graphene oxide

GOS:

Galacto-oligosaccharides

IβG:

Immobilized β-galactosidase

SβG:

Soluble β-galactosidase

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Acknowledgments

The authors are thankful to Prof. Waseem Ahmad (Center of Excellence in Genomic and Medicine Research, King Abdulaziz University, Saudi Arabia) for going through the manuscript critically and suggesting relevant changes.

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Authors and Affiliations

  1. Department of Biochemistry, Ibn Sina National College for Medical Studies, Jeddah, Kingdom of Saudi Arabia

    Rukhsana Satar

  2. Department of Basic Sciences, Ibn Sina National College for Medical Studies, Jeddah, Kingdom of Saudi Arabia

    Syed Ahmed Ismail

  3. King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia

    Mohd Rehan

  4. Center of Excellence in Genomic and Medicine Research, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia

    Shakeel Ahmed Ansari

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  1. Rukhsana Satar
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  2. Syed Ahmed Ismail
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  4. Shakeel Ahmed Ansari
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Correspondence to Shakeel Ahmed Ansari.

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Satar, R., Ismail, S.A., Rehan, M. et al. Elucidating the binding efficacy of β-galactosidase on graphene by docking approach and its potential application in galacto-oligosaccharide production. Bioprocess Biosyst Eng 39, 807–814 (2016). https://doi.org/10.1007/s00449-016-1560-6

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  • DOI: https://doi.org/10.1007/s00449-016-1560-6

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