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Production of ε-poly-l-lysine by Streptomyces sp. using resin-based, in situ product removal

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Abstract

Resin-based, in situ product removal (ISPR) was used to increase production of ε-poly-l-lysine (PL) by Streptomyces sp. GIM8. D152 resin was selected over Amberlite IRC-50, Amberlite IRC-76 and Amberlite IR-120 to develop ISPR using adsorption capacity and desorption ratio as bases. The yield of PL in response to external PL was unaffected in shake-flask culture; however, the production of PL increased to 2.9 from 0.8 g l−1 shake-flasks using ISPR. In a 5 l fermentor, 23.4 g PL l−1 was achieved compared to 3.76 g PL l−1, in the controls by attaching two bags of D152 resin to the probes and baffles of the fermentor.

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Acknowledgments

This work was supported by the Program for Industry-Academia-Research (2009B090300300), the Guangdong-Hongkong Technology Cooperation Funding (2007A020902003), and the Science and Technology Planning Project of Guangdong Province, China (2008A010900003 and 2009B011300004).

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Authors and Affiliations

  1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China

    Shengrong Liu

  2. Guangdong Institute of Microbiology, Guangzhou, 510070, People’s Republic of China

    Shengrong Liu, Qingping Wu, Jumei Zhang & Shuping Mo

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  1. Shengrong Liu
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  2. Qingping Wu
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  3. Jumei Zhang
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  4. Shuping Mo
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Correspondence to Qingping Wu.

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Liu, S., Wu, Q., Zhang, J. et al. Production of ε-poly-l-lysine by Streptomyces sp. using resin-based, in situ product removal. Biotechnol Lett 33, 1581–1585 (2011). https://doi.org/10.1007/s10529-011-0616-6

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  • DOI: https://doi.org/10.1007/s10529-011-0616-6

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