Skip to main content
SpringerLink
Log in

Citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 and purification of citric acid

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

In this study, citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 was investigated. After the compositions of the extract of Jerusalem artichoke tubers for citric acid production were optimized, the results showed that natural components of extract of Jerusalem artichoke tubers without addition of any other components were suitable for citric acid production by the yeast strain. During 10 L fermentation using the extract containing 84.3 g L−1 total sugars, 68.3 g L−1 citric acid was produced and the yield of citric acid was 0.91 g g−1 within 336 h. At the end of the fermentation, 9.2 g L−1 of residual total sugar and 2.1 g L−1 of reducing sugar were left in the fermented medium. At the same time, citric acid in the supernatant of the culture was purified. It was found that 67.2 % of the citric acid in the supernatant of the culture was recovered and purity of citric acid in the crystal was 96 %.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Alvarez-Vasquez F, González-Alcón C, Torres NV (2000) Metabolism of citric acid production by Aspergillus niger: model definition, steady-state analysis and constrained optimization of citric acid production rate. Biotechnol Bioeng 70:82–108

    Article  CAS  Google Scholar 

  2. Imandi SB, Bandaru VVR, Somalanka SR, Gara HR (2007) Optimization of medium constituents for the production of citric acid from byproduct glycerol using Doehlert experimental design. Enzym Microb Technol 40:1367–1371

    Article  CAS  Google Scholar 

  3. Liu XY, Chi Z, Liu GL, Wang F, Madzak C, Chi ZM (2010) Inulin hydrolysis and citric acid production from inulin using the surface-engineered Yarrowia lipolytica displaying inulinase. Metab Eng 12:469–476

    Article  CAS  Google Scholar 

  4. Zhao CH, Cui W, Liu XY, Chi ZM, Madzak C (2010) Expression of inulinase gene in the oleaginous yeast Yarrowia lipolytica and single cell oil production from inulin-containing materials. Metab Eng 12:510–517

    Article  CAS  Google Scholar 

  5. Förster A, Aurich A, Mauersberger S, Barth G (2007) Citric acid production from sucrose using a recombinant strain of the yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 75:1409–1417

    Article  Google Scholar 

  6. Chi ZM, Zhang T, Cao TS, Liu XY, Cui W, Zhao CH (2011) Biotechnological potential of inulin for bioprocesses. Biores Technol 10:24295–24303

    Google Scholar 

  7. Papanikolaou S, Galiotou-Panayotou M, Fakas S, Komaitis M, Aggeli G (2008) Citric acid production by Yarrowia lipolytica cultivated on olive-mill waste water-based media. Biores Technol 99:2419–2428

    Article  CAS  Google Scholar 

  8. Liu XY, Chi Z Z, Liu GL, Madzak C, Chi ZM (2013) Both decrease in ACL1 gene expression and increase in ICL1 gene expression in marine-derived yeas Yarrowia lipolytica expressing INU1 gene enhance citric acid production from inulin. Mar Biotechnol 15:26–36

    Article  CAS  Google Scholar 

  9. Gong F, Sheng J, Chi ZM, Li J (2007) Inulinase production by a marine yeast Pichia guilliermondii and inulin hydrolysis by the crude inulinase. J Ind Microbiol Biotechnol 34:179–185

    Article  CAS  Google Scholar 

  10. Camp BJ, Farmer L (1967) A rapid spectrophotometric method for the determination of citric acid in blood. Clin Chem 13:501–505

    CAS  Google Scholar 

  11. Spiro RG (1966) Analysis of sugars found in glycoproteins. Meth Enzymol 8:3–26

    Article  CAS  Google Scholar 

  12. Chi ZM, Liu J, Zhang W (2001) Trehalose accumulation from soluble starch by Saccharomycopsis fibuligera sdu. Enzym Microb Technol 28:240–245

    Article  CAS  Google Scholar 

  13. Sanchez-Riera F (2010) Production of organic acids. Biotechnol 5:1–9

    Google Scholar 

  14. Papagianni M (2007) Advances in citric acid fermentation by Aspergillus niger: biochemical aspects, membrane transport and modeling. Biotechnol Adv 25:244–263

    Article  CAS  Google Scholar 

  15. Kamzolova SV, Morgunov IG, Aurich A, Perevoznikova OA, Shishkanova NV, Stottmeister U, Finogenova TV (2005) Lipase secretion and citric acid production in Yarrowia lipolytica yeast grown on animal and vegetable fat. Food Technol Biotechnol 43:113–122

    CAS  Google Scholar 

  16. Klasson TK, Clausen EC, Gaddy JL (1989) Continuous fermentation for the production of citric acid from glucose. Appl Biochem Biotechnol 491:491–509

    Article  Google Scholar 

  17. Papanikolaou S, Chatzifragkou A, Fakas S, Galiotou-Panayotou M, Komaitis M, Nicaud JM, Aggelis G (2009) Biosynthesis of lipids and organic acids by Yarrowia lipolytica strains cultivated on glucose. Eur J Lipid Sci Technol 111:1221–1232

    Article  CAS  Google Scholar 

  18. Angumeenal AR, Venkappayya D (2012) An overview of citric acid production. LWT-Food Sci Technol 22:1–4

    Google Scholar 

  19. Papagianni M, Mattey Berovic MM, Kristiansen B (1999) Aspergillus niger morphology and citric acid production in submerged batch fermentation: effects of culture pH, phosphate and manganese levels. Food Technol Biotechnol 37:165–171

    CAS  Google Scholar 

  20. Kubicek CP, Röhr M (1977) Influence of manganese on enzyme synthesis and citric acid accumulation in Aspergillus niger. Appl Microbiol Biotechnol 4:167–175

    CAS  Google Scholar 

  21. Crolla A, Kennedy KJ (2001) Optimization of citric acid production from Candida lipolytica Y-1095 using n-paraffin. J Biotechnol 89:27–40

    Article  CAS  Google Scholar 

  22. Papanikolaou S, Galiotou-Panayotou M, Chevalot I, Komaitis M, Marc I, Aggelis G (2006) Influence of glucose and saturated free fatty acid mixtures on citric acid and lipid production by Yarrowia lipolytica. Curr Microbiol 52:134–142

    Article  CAS  Google Scholar 

  23. Majumda L, Khalid I, Munshi MK, Alam K, Or-Rashid H (2010) Citric acid production by Aspergillus niger using molasses and pumpkin as substrates. Eur J Biol Sci 2:1–8

    Google Scholar 

  24. Wojtatowicz M, Rymowicz W, Kautola H (1991) Comparison of different strains of the yeast Yarrowia lipolgtica for citric acid production from glucose hydrol. Appl Biochem Biotechnol 31:165–174

    Article  CAS  Google Scholar 

  25. Moeller L, Grunberg M, Zehnsdorf A, Strehlitz B, Bley T (2010) Biosensor online control of citric acid production from glucose by Yarrowia lipolytica using semicontinuous fermentation. Eng Life Sci 10:311–320

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by Grant 31070029 from the National Natural Science Foundation of China and Hi-Tech Research and Development Program of China (863) (Grant No. 2012AA021205).

Author information

Authors and Affiliations

  1. Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, Shandong, 266003, China

    Ling-Fei Wang, Zhi-Peng Wang, Xiao-Yan Liu & Zhen-Ming Chi

Authors
  1. Ling-Fei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhi-Peng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiao-Yan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhen-Ming Chi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhen-Ming Chi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, LF., Wang, ZP., Liu, XY. et al. Citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 and purification of citric acid. Bioprocess Biosyst Eng 36, 1759–1766 (2013). https://doi.org/10.1007/s00449-013-0951-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-013-0951-1

Keywords

Search

Navigation