Skip to main content
Log in

Comparison of different strains of the yeastYarrowia lipolytica for citric acid production from glucose hydrol

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Four commercial strains and two mutants of the yeast speciesYarrowia lipolytica were screened using batch fermentation. StrainY.lipolytica A-101-1.14 (induced with UV irradiation) was found to be the most suitable for citric acid production from glucose hydrol (39.9% glucose and 2.1% other sugars), a byproduct of glucose production from potato starch. The specific rate of total citric and isocitric acid production was 0.138 g/g.h, the yield on consumed glucose 0.93 g/g, and the productivity achieved was as high as 1.25 g/L.h All of the tested yeast strains were able to utilize only the glucose from the glucose hydrol medium. Thus, some residual higher oligosaccharides remained in the process effluent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Marchal, R., Chaude, O., and Metche, M. M. (1977),Eur. J. Appl. Microbiol. 4, 111–123.

    Article  CAS  Google Scholar 

  2. Behrens, U., Weissbrodt, E., and Lehmann, W. (1978),Z. Allg. Mikrobiol. 18, 549–558.

    Article  CAS  Google Scholar 

  3. Treton, B., Le Dall, M. T., and Heslot, H. (1978),Eur. J. Appl. Microbiol. Bio technol. 6, 67–77.

    Article  CAS  Google Scholar 

  4. Finogenova, T. V., Grinchak, A. V., Illarianova, V. J., and Shishkanova, N. V. (1979),Prikl. Biokhim. Mikrobiol. 15, 811–816.

    CAS  Google Scholar 

  5. Enzminger, J. D., and Asenjo, J. A. (1986),Biotechnol. Lett. 8, 7–12.

    Article  CAS  Google Scholar 

  6. Briffaud, J., and Engasser, J. M. (1979),Biotechnol. Bioeng. 21, 2093–2111.

    Article  CAS  Google Scholar 

  7. Stottmeister, U. (1979),Z. Allg. Mikrobiol. 19, 763–765.

    Article  CAS  Google Scholar 

  8. Maddox, J., and Kingston, P. (1983),Biotechnol. Lett. 5, 795–798.

    Article  CAS  Google Scholar 

  9. Ishi, K., Nakajima, Y., and Iwakura, T. (1972),Ger. Pat. 2,157,847.

    Google Scholar 

  10. Miall, M., and Parker, G. F. (1975),Ger. Pat. 2,429,224.

    Google Scholar 

  11. Liu, Y. T. (1975),Taiwan Tang Yeh Yen Chiu So Yen Chiu Hui Pao 68, 55, 56.

    Google Scholar 

  12. Van der Walt, J. P., and von Arx, J. A. (1980),Antonie van Leeuwenhoek 46, 517–521.

    Article  Google Scholar 

  13. Barth, G., and Weber, H. (1983),Z. Allg. Mikrobiol. 23, 147–157.

    Article  CAS  Google Scholar 

  14. Stern, J. (1957),Methods in Enzymology, vol. 3, Colowick, S., and Kaplan, N., eds., Academic, New York, pp. 425–431.

    Google Scholar 

  15. Goldberg, D. M., and Ellis, G. (1983),Methods of Enzymatic Analysis, vol. 3, Bergmeyer, H. U., ed., Verlag Chemie GmbH, Weinheim, Germany, pp. 183–190.

    Google Scholar 

  16. Briffaud, J., and Engasser, J. M. (1979),Biotechnol. Bioeng. 21, 2083–2092.

    Article  CAS  Google Scholar 

  17. Behrens, U., Thirsch, A., Weissbrodt, E., and Stottmeister, U. (1987),Acta Biotechnol. 7, 179–183.

    Article  CAS  Google Scholar 

  18. Moresi, M., Cimarelli, D., Gasparrini, G., Liuzzo, G., and Marinelli, R. (1980),J. Chem. Technol. Biotechnol. 30, 266–277.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wojtatowicz, M., Rymowicz, W. & Kautola, H. Comparison of different strains of the yeastYarrowia lipolytica for citric acid production from glucose hydrol. Appl Biochem Biotechnol 31, 165–174 (1991). https://doi.org/10.1007/BF02921787

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02921787

Index Entries

Navigation