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Identification and characterization of Klebsiella pneumoniae aldehyde dehydrogenases increasing production of 3-hydroxypropionic acid from glycerol

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Abstract

Klebsiella pneumoniae produces 3-hydroxypropionic acid (3-HP) from glycerol with oxidation of 3-hydroxypropionaldehyde (3-HPA) to 3-HP in a reaction catalyzed by aldehyde dehydrogenase (ALDH). In the present study, two putative ALDHs of K. pneumoniae, YneI and YdcW were identified and characterized. Recombinant YneI was specifically active on 3-HPA and preferred NAD+ as a cofactor, whereas YdcW exhibited broad substrate specificity and preferred NADP+ as a cofactor. Overexpression of ALDHs in the glycerol oxidative pathway-deficient mutant K. pneumoniae AK resulted in a significant increase in 3-HP production upon shake-flask culture. The final titers of 3-HP were 2.4 and 1.8 g L−1 by recombinants overexpressing YneI and YdcW, respectively. Deletion of the ALDH gene from K. pneumoniae did not affect the extent of 3-HP synthesis, implying non-specific activity of ALDHs on 3-HPA. The ALDHs might play major roles in detoxifying the aldehyde generated in glycerol metabolism.

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Acknowledgments

The authors are very grateful to Prof. Sunghoon Park for the kind gift of 3-hydroxypropionaldehyde. This study was supported by the Ministry of Education, Science and Technology, Korea.

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Authors and Affiliations

  1. Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si, 580-185, South Korea

    Lian Hua Luo, Jeong-Woo Seo, Sun-Yeon Heo, Baek-Rock Oh & Chul Ho Kim

  2. Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University, Jeonju-si, 561-756, South Korea

    Lian Hua Luo & Dae-Hyuk Kim

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  1. Lian Hua Luo
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  2. Jeong-Woo Seo
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  3. Sun-Yeon Heo
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  5. Dae-Hyuk Kim
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  6. Chul Ho Kim
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Correspondence to Chul Ho Kim.

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Luo, L.H., Seo, JW., Heo, SY. et al. Identification and characterization of Klebsiella pneumoniae aldehyde dehydrogenases increasing production of 3-hydroxypropionic acid from glycerol. Bioprocess Biosyst Eng 36, 1319–1326 (2013). https://doi.org/10.1007/s00449-012-0880-4

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  • DOI: https://doi.org/10.1007/s00449-012-0880-4

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