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Ultrastructural localization of vimentin immunoreactivity and gene expression in tanycytes and their alterations in hamsters kept under different photoperiods

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Abstract

Tanycytes are located in the basolateral walls of the third ventricle. By light- and electron-microscopic immunohistochemistry, we demonstrated that the tanycytes of Djungarian hamsters were intensely immunostained with the vimentin monoclonal antibody V9. The cells always extended long radial processes in which aggregations of vimentin-immunoreactive intermediate filaments were prominent. The tanycytes showed photoperiod-dependent changes. The population of vimentin-immunoreactive tanycytes was increased in hamsters exposed to continuous lighting for 1 month or to a long photoperiod (16 h light:8 h dark) for 2 months. On the other hand, the immunoreactive cells were significantly decreased in hamsters exposed to complete darkness for 1 month or to a short photoperiod (8 h light:16 h dark) for 2 months. The pericapillary spaces of the primary plexus of the portal circulation system were lined by the end-feet of tanycytic processes. Electron microscopy confirmed that the tanycytic processes were markedly decreased in number and size after exposure to complete darkness. Expression of vimentin mRNA in the hamster mediobasal hypothalamus was detected by the reverse transcription-polymerase chain reaction (RT-PCR). The alterations of vimentin mRNA expression under different photoperiods were analyzed using laser capture microdissection and real-time quantitative PCR. The level of vimentin mRNA in the mediobasal hypothalamus was enhanced after exposure to continuous lighting for 1 month or to a long photoperiod for 2 months, whereas it was significantly diminished after exposure to constant darkness or short photoperiod. These changes in the tanycytes under different photoperiods may influence the portal circulation system and also the cell activity of the pars tuberalis, and may thus participate in photoperiodic regulation of the endocrine system.

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References

  • Aleandri V, Spina V, Morini A (1996) The pineal gland and reproduction. Hum Reprod Update 2:225–235

    CAS  PubMed  Google Scholar 

  • Blazquez JL, Guerra M, Pastor F, Peruzzo B, Amat P, Rodriguez EM (2002) Antibodies obtained by xenotransplantation of organ-cultured median eminence specifically recognize hypothalamic tanycytes. Cell Tissue Res 308:241–253

    Article  CAS  PubMed  Google Scholar 

  • Bockmann J, Böckers TM, Vennemann B, Niklowitz P, Müller J, Wittkowski W, Sabel B, Kreutz MR (1996) Short photoperiod-dependent down-regulation of thyrotropin-α and -β in hamster pars tuberalis-specific cells is prevented by pinealectomy. Endocrinology 137:1804–1813

    CAS  PubMed  Google Scholar 

  • Bovolenta P, Liem RK, Mason CA (1984) Development of cerebellar astroglia: transitions in form and cytoskeletal content. Dev Biol 102:248–259

    CAS  PubMed  Google Scholar 

  • Chouaf L, Didier-Bazes M, Aguera M, Tardy M, Sallanon M, Kitahama K, Belin MF (1989) Comparative marker analysis of the ependymocytes of the subcommissural organ in four different mammalian species. Cell Tissue Res 257:255–262

    CAS  PubMed  Google Scholar 

  • Devries MJ, Ferreira SA, Glass JD (1989) Evidence that short photoperiod-induced gonadal regression in the Mongolian gerbil is mediated by the action of melatonin in the medial hypothalamus. Brain Res 494:241–246

    Article  CAS  PubMed  Google Scholar 

  • Duncan MJ, Davis FC (1993) Developmental appearance and age related changes in specific 2-[125I]iodomelatonin binding sites in the suprachiasmatic nuclei of female Syrian hamsters. Dev Brain Res 73:205–212

    CAS  Google Scholar 

  • Elmquist JK, Swanson JJ, Sakaguchi DS, Ross LR, Jacobson CD (1994) Developmental distribution of GFAP and vimentin in the Brazilian opossum brain. J Comp Neurol 344:283–296

    CAS  PubMed  Google Scholar 

  • Flament-Durand J, Brion JP (1985) Tanycytes: morphology and functions: a review. Int Rev Cytol 96:121–155

    CAS  PubMed  Google Scholar 

  • Gauer F, Masson-Pévet M, Pévet P (1992) Pinealectomy and constant illumination increase the density of melatonin binding sites in the pars tuberalis of rodents. Brain Res 575:32–38

    Article  CAS  PubMed  Google Scholar 

  • Hastings MH, Walker AP, Roberts AC, Herbert J (1988) Intra-hypothalamic melatonin blocks photoperiodic responsiveness in the male Syrian hamster. Neuroscience 24:987–991

    Article  CAS  PubMed  Google Scholar 

  • Kameda Y (1996) Immunoelectron microscopic localization of vimentin in sustentacular cells of the carotid body and the adrenal medulla of guinea pigs. J Histochem Cytochem 44:1439–1449

    CAS  PubMed  Google Scholar 

  • Kameda Y, Miura M, Ohno S (2000a) Expression of the common α-subunit mRNA of glycoprotein hormones during the chick pituitary organogenesis, with special reference to the pars tuberalis. Cell Tissue Res 299:71–80

    CAS  PubMed  Google Scholar 

  • Kameda Y, Miura M, Ohno S (2000b) Expression and development of the proenkephalin mRNA in the C cells of chicken ultimobranchial glands. Brain Res 852:453–462

    CAS  PubMed  Google Scholar 

  • Kameda Y, Miura M, Nishimaki T (2001) Effect of different photoperiods on the ultrastructure of the specific secretory cells and α-subunit mRNA level in the chicken pars tuberalis. Cell Tissue Res 304:111–120

    CAS  PubMed  Google Scholar 

  • Kobayashi H, Wada M, Uemura H, Ueck M (1972) Uptake of peroxidase from the third ventricle by ependymal cells of the median eminence. Z Zellforsch 127:545–551

    CAS  Google Scholar 

  • Kozlowski GP, Coates PW (1985) Ependymoneuronal specializations between LHRH fibers and cells of the cerebroventricular system. Cell Tissue Res 242:301–311

    CAS  PubMed  Google Scholar 

  • Langub MC, Watson RE (1992) Estrogen receptor-immunoreactive glia, endothelia, and ependyma in guinea pig preoptic area and median eminence: electron microscopy. Endocrinology 130:364–372

    CAS  PubMed  Google Scholar 

  • Lerchl A, Schlatt S (1993) Influence of photoperiod on pineal melatonin synthesis, fur color, body weight, and reproductive function in the female Djungarian hamster, Phodopus sungorus. Neuroendocrinology 57:359–364

    CAS  PubMed  Google Scholar 

  • Ma YJ, Junier MP, Costa ME, Ojeda SR (1992) Transforming growth factor-α gene expression in the hypothalamus is developmentally regulated and linked to sexual maturation. Neuron 9:657–670

    CAS  PubMed  Google Scholar 

  • Malpaux B, Daveau A, Maurice F, Gayrard V, Thiery JC (1993) Short-day effects of melatonin on luteinizing hormone secretion in the ewe: evidence for central sites of action in the mediobasal hypothalamus. Biol Reprod 48:752–760

    CAS  PubMed  Google Scholar 

  • Maywood ES, Hastings MH (1995) Lesions of the iodomelatonin-binding sites of the mediobasal hypothalamus spare the lactotropic, but block the gonadotropic response of male Syrian hamsters to short photoperiod and to melatonin. Endocrinology 136:144–153

    CAS  PubMed  Google Scholar 

  • Meddle SL, Follett BK (1995) Photoperiodic activation of Fos-like immunoreactive protein in neurones within the tuberal hypothalamus of Japanese quail. J Comp Physiol 176:79–89

    CAS  Google Scholar 

  • Merchant RE, Dollar JR (1981) The median eminence in normal, ovariectomized, and ovariectomized-estradiol-treated hamsters: an ultrastructural study. Am J Anat 160:1–16

    CAS  PubMed  Google Scholar 

  • Messager S, Ross AW, Barrett P, Morgan PJ (1999) Decoding photoperiodic time through Per1 and ICER gene amplitude. Proc Natl Acad Sci U S A 96:9938–9943

    CAS  PubMed  Google Scholar 

  • Morgan PJ, Ross AW, Graham ES, Adam C, Messager S, Barrett P (1998) oPer1 is an early response gene under photoperiodic regulation in the ovine pars tuberalis. J Neuroendocrinol 10:319–323

    CAS  PubMed  Google Scholar 

  • Nuesslein-Hildesheim B, O'Brien JA, Ebling FJ, Maywood ES, Hastings MH (2000) The circadian cycle of mPER clock gene products in the suprachiasmatic nucleus of the Siberian hamster encodes both daily and seasonal time. Eur J Neurosci 12:2856–2864

    Article  CAS  PubMed  Google Scholar 

  • Ojeda SR, Ma YJ, Lee BJ, Prevot V (2000) Glia-to-neuron signaling and the neuroendocrine control of female puberty. Rec Progr Horm Res 55:197–223

    CAS  PubMed  Google Scholar 

  • Prevot V, Croix D, Bouret S, Dutoit S, Tramu G, Stefano GB, Beauvillain JC (1999) Definitive evidence for the existence of morphological plasticity in the external zone of the median eminence during the rat estrous cycle: implication of neuro-glio-endothelial interactions in gonadotropin-releasing hormone release. Neuroscience 94:809–819

    Article  CAS  PubMed  Google Scholar 

  • Pulido-Caballero J, Jiménez-Sampedro F, Echevarria-Aza D, Martinez-Millán L (1994) Postnatal development of vimentin-positive cells in the rabbit superior colliculus. J Comp Neurol 343:102–112

    CAS  PubMed  Google Scholar 

  • Reppert SM, Weaver DR, Ebisawa T (1994) Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. Neuron 13:1177–1185

    CAS  PubMed  Google Scholar 

  • Reppert SM, Godson C, Mahle CD, Weaver DR, Slaugenhaupt SA, Gusella JF (1995) Molecular characterization of a second melatonin receptor expressed in human retina and brain: the Mel1b melatonin receptor. Proc Natl Acad Sci U S A 92:8734–8738

    CAS  PubMed  Google Scholar 

  • Rodriguez EM, Gonzalez CB, Delannoy L (1979) Cellular organization of the lateral and postinfundibular regions of the median eminence in the rat. Cell Tissue Res 201:377–408

    PubMed  Google Scholar 

  • Roy D, Angelini NL, Fujieda H, Brown GM, Belsham DD (2001) Cyclical regulation of GnRH gene expression in GT1–7 GnRH-secreting neurons by melatonin. Endocrinology 142:4711–4720

    CAS  PubMed  Google Scholar 

  • Sancho-Tello M, Valles S, Montoliu C, Renau-Piqueras J, Guerri C (1995) Developmental pattern of GFAP and vimentin gene expression in rat brain and in radial glial cultures. Glia 15:157–166

    CAS  PubMed  Google Scholar 

  • Stoeckel ME, Hindelang C, Klein MJ, Poissonnier M, Félix JM (1994) Expression of the α-subunit of glycoprotein hormones in the pars tuberalis-specific glandular cells in rat, mouse and guinea-pig. Cell Tissue Res 278:617–624

    CAS  PubMed  Google Scholar 

  • Sun ZS, Albrecht U, Zhuchenko O, Bailey J, Eichele G, Lee CC (1997) RIGUI, a putative mammalian ortholog of the Drosophila period gene. Cell 90:1003–1011

    CAS  PubMed  Google Scholar 

  • Thomas MA, Hauptfleisch S, Fleissner G, Lemmer B (2003) Localization of angiotensin II (AT1)-receptor-immunoreactive fibers in the hypothalamus of rats: angiotensin II-sensitive tanycytes in the ependyma of the third ventricle? Brain Res 967:281–284

    Article  CAS  PubMed  Google Scholar 

  • Von Gall C, Garabette ML, Kell CA, Frenzel S, Dehghani F, Schumm-Draeger PM, Weaver DR, Korf HW, Hastings MH, Stehle JH (2002) Rhythmic gene expression in pituitary depends on heterologous sensitization by the neurohormone melatonin. Nature Neurosci 5:234–238

    Article  PubMed  Google Scholar 

  • Weaver DR, Rivkees SA, Reppert SM (1989) Localization and characterization of melatonin receptors in rodent brain by in vitro autoradiography. J Neurosci 9:2581–2590

    CAS  PubMed  Google Scholar 

  • Williams LM, Morgan PJ (1988) Demonstration of melatonin-binding sites on the pars tuberalis of the rat. J Endocrinol 119:R1–R3

    CAS  PubMed  Google Scholar 

  • Wittkowski W, Bockmann J, Kreutz MR, Böckers TM (1999) Cell and molecular biology of the pars tuberalis of the pituitary. Int Rev Cytol 185:157–194

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank the members of the Electron Microscope Laboratory Center, Kitasato University School of Medicine, for their expert technical contributions.

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  1. Department of Anatomy, Kitasato University School of Medicine, Sagamihara, 228-8555, Kanagawa, Japan

    Yoko Kameda, Yuta Arai & Toshiyuki Nishimaki

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  1. Yoko Kameda
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  2. Yuta Arai
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  3. Toshiyuki Nishimaki
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Correspondence to Yoko Kameda.

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Kameda, Y., Arai, Y. & Nishimaki, T. Ultrastructural localization of vimentin immunoreactivity and gene expression in tanycytes and their alterations in hamsters kept under different photoperiods. Cell Tissue Res 314, 251–262 (2003). https://doi.org/10.1007/s00441-003-0789-y

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