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Tandem organization of StarkB element (22.8 kb) in the maize B chromosome

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Abstract

A very large repetitive element (StarkB, 22.8 kb) is present in the maize B chromosome, presumably not organized as tandem arrays. Results of the current study are contrary to this notion. Out of eighteen StarkB-carrying sequences, nine were the expected internal fragment of StarkB, and nine others were fragments spanning two StarkB elements. One of the two StarkB components, GrandeB, was flanked in all clones with identical target sequences, as opposed to other Grandes that are associated with different target sequences. Also observed was a prominent Southern signal associated with a fragment representing the junction of two adjacent StarkB units. A clone possessing a structure inverse to that of the second component of StarkB is proposed to be the initial element into which a GrandeB inserted to derive StarkB. Most, if not all, isolated StarkB arrays were not the original form, being disrupted by the invasion of various mobile elements intertwined with various stages of amplification.

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Author notes

  1. Shu-fen Peng

    Present address: Department of Chemical Engineering, National Tsing Hua University, Hsinchu, 300, Taiwan, ROC

Authors and Affiliations

  1. Institute of Molecular Biology, National Chung Hsing University, Taichung, 402, Taiwan, ROC

    Kuan-lin Lo, Shu-fen Peng, Liang-jwu Chen & Bor-yaw Lin

Authors
  1. Kuan-lin Lo
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  2. Shu-fen Peng
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  3. Liang-jwu Chen
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  4. Bor-yaw Lin
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Corresponding author

Correspondence to Bor-yaw Lin.

Additional information

Communicated by M. Grandbastien.

Nucleotide sequence data reported are available in the GenBank database under the accession numbers EF468500 to EF468511, EU528676, and FJ386410 to FJ386429.

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Lo, Kl., Peng, Sf., Chen, Lj. et al. Tandem organization of StarkB element (22.8 kb) in the maize B chromosome. Mol Genet Genomics 282, 131–139 (2009). https://doi.org/10.1007/s00438-009-0452-8

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  • DOI: https://doi.org/10.1007/s00438-009-0452-8

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