Abstract
The overall composition of the maize B is similar to that of the standard chromosome complement (A-chromosomes). This has been demonstrated by the use of several methods including: (a) genomicin situ hybridization (GISH), (b) analysis of highly repetitive sequences by the comparison of restriction digests of 0B and +B DNA and (c) the characterization of middle-repetitive cloned sequences. By the use of the technique of random amplification of polymorphic DNA-polymerase chain reaction, we have identified a B-specific repetitive sequence family. Sequence analysis of the B-specific clone reveals a relationship to the PREM-1 family of maize retroelements, which are preferentially transcribed in pollen. These results suggest an internal origin of the B-chromosome from within the maize genome, but demonstrate also that specific sequences can evolve by rapid processes of genomic turnover. Models for the origin of the maize B are discussed in the context of the present data.
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accepted for publication by J. S. (Pat) Heslop-Harrison
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Stark, E.A., Connerton, I., Bennett, S.T. et al. Molecular analysis of the structure of the maize B-chromosome. Chromosome Res 4, 15–23 (1996). https://doi.org/10.1007/BF02254939
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DOI: https://doi.org/10.1007/BF02254939