Abstract
In this study, two melon bacterial artificial chromosome (BAC) clones have been sequenced and annotated. BAC 1-21-10 spans 92 kb and contains the nsv locus conferring resistance to the Melon Necrotic Spot Virus (MNSV) in melon linkage group 11. BAC 13J4 spans 98 kb and belongs to a BAC contig containing resistance gene homologues, extending a previous sequenced region of 117 kb in linkage group 4. Both regions have microsyntenic relationships to the model plant species Arabidopsis thaliana, and to Medicago truncatula and Populus trichocarpa. The network of synteny found between melon and each of the sequenced genomes reflects the polyploid structure of Arabidopsis, Populus, and Medicago genomes due to whole genome duplications (WGD). A detailed analysis revealed that both melon regions have a lower relative syntenic quality with Arabidopsis (eurosid II) than when compared to Populus and Medicago (eurosid I). Although phylogenetically Cucurbitales seem to be closer to Fabales than to Malphigiales, synteny was higher between both melon regions and Populus. Presented data imply that the recently completed Populus genome sequence could preferentially be used to obtain positional information in melon, based on microsynteny.
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Acknowledgments
The authors thank Mercè Miquel from the Sequencing Service of IBMB-CSIC (Barcelona, Spain) for the sequencing of shotgun clones. W. D. is recipient of a postdoctoral contract from the Centre de Recerca en Agrigenòmica (CRAG).
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Communicated by Y. Van de Peer.
Wim Deleu and Víctor González contributed equally to this work. The nucleotide sequences of BACs 1-21-10 and 13J4 are available in the DDBJ/EMBL/GenBank databases under the accession numbers EF188258 and EF657230, respectively.
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Deleu, W., González, V., Monfort, A. et al. Structure of two melon regions reveals high microsynteny with sequenced plant species. Mol Genet Genomics 278, 611–622 (2007). https://doi.org/10.1007/s00438-007-0277-2
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DOI: https://doi.org/10.1007/s00438-007-0277-2