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Analysis of the melon genome in regions encompassing TIR-NBS-LRR resistance genes

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Abstract

Plant genomes contain numerous genes (R-genes) that play a role in initiating defence measures against their particular pathogens. Defence mechanisms controlled by R-genes have been the focus of extensive research over the past several years. The majority of the R-genes described so far belong to a super-family of genes (150–600 members) that encode proteins with a nucleotide binding site (NBS), some leucine-rich repeats (LRR) and an N-terminal domain that shows similarity to the Toll and Interleukin-1 receptors (TIR) or a N-terminal coiled-coil (CC) domain. Analysis of four regions of the melon (Cucumis melo) genome, including two sequenced BACs, identified 14 TIR–NBS–LRR genes. Known disease resistance genes have been mapped in three of these regions. Transcriptional expression was detected for predicted genes that are possibly involved in defence responses to pathogen attack. TIR–NBS–LRR genes appear to be clustered in the melon genome. They contain all the conserved motifs that have previously been described for their counterparts in other species, although differences were also detected. The results presented here may contribute to a better understanding of the genomic distribution and evolution of this group of resistance gene homologues and their variability.

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Acknowledgements

The authors would like to thank Mercé Miquel from the IBMB Sequencing Service for the DNA sequencing, Montserrat Martin for providing the TRV infected melon leaves, and David Lightfoot for providing much useful discussion and helpful comments on the manuscript. Funds for this project were provided by Grant No. 2FD97-0286-(02–01) from the Comisión Interdepartamental de Ciencia y Tecnologia (CICYT) and Grant No. 2000FI-00316 to Hans van Leeuwen from the Comissió Interdepartamental de Recerca i Tecnologia (CIRIT). This work was carried out within the context of the Centre de Referencia de Biotecnologia (CERBA) of the Generalitat de Catalunya.

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Authors and Affiliations

  1. Laboratori de Genética Molecular Vegetal, Consorci CSIC-IRTA, Barcelona, Spain

    Hans van Leeuwen, Jordi Garcia-Mas, María Coca, Pere Puigdoménech & Amparo Monfort

  2. Departament de Genética Molecular, Jordi Girona 18, 08034, Barcelona, Spain

    Hans van Leeuwen, María Coca & Pere Puigdoménech

  3. Departament de Genética Vegetal, Institut de Recerca i Tecnologia Agroalimentáries (IRTA), Ctra. de Cabrils s/n, 08348, Cabrils, Barcelona, Spain

    Jordi Garcia-Mas & Amparo Monfort

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  1. Hans van Leeuwen
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  2. Jordi Garcia-Mas
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  3. María Coca
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  4. Pere Puigdoménech
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  5. Amparo Monfort
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Correspondence to Amparo Monfort.

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Communicated by M.-A. Grandbastien

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van Leeuwen, H., Garcia-Mas, J., Coca, M. et al. Analysis of the melon genome in regions encompassing TIR-NBS-LRR resistance genes. Mol Genet Genomics 273, 240–251 (2005). https://doi.org/10.1007/s00438-004-1104-7

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  • DOI: https://doi.org/10.1007/s00438-004-1104-7

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