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Retand: a novel family of gypsy-like retrotransposons harboring an amplified tandem repeat

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Abstract

In this paper we describe a pair of novel Ty3/gypsy retrotransposons isolated from the dioecious plant Silene latifolia, consisting of a non-autonomous element Retand-1 (3.7 kb) and its autonomous partner Retand-2 (11.1 kb). These two elements have highly similar long terminal repeat (LTR) sequences but differ in the presence of the typical retroelement coding regions (gag-pol genes), most of which are missing in Retand-1. Moreover, Retand-2 contains two additional open reading frames in antisense orientation localized between the pol gene and right LTR. Retand transcripts were detected in all organs tested (leaves, flower buds and roots) which, together with the high sequence similarity of LTRs in individual elements, indicates their recent transpositional activity. The autonomous elements are similarly abundant (2,700 copies) as non-autonomous ones (2,100 copies) in S. latifolia genome. Retand elements are also present in other Silene species, mostly in subtelomeric heterochromatin regions of all chromosomes. The only exception is the subtelomere of the short arm of the Y chromosome in S. latifolia which is known to lack the terminal heterochromatin. An interesting feature of the Retand elements is the presence of a tandem repeat sequence, which is more amplified in the non-autonomous Retand-1.

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Acknowledgments

This work was supported by the Czech Science Foundation (grant nos. 204/05/2097, 204/05/H505 and 204/04/1207) and AVOZ50510513 from the Academy of Sciences of the Czech Republic.

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Authors and Affiliations

  1. Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65, Brno, Czech Republic

    Eduard Kejnovsky, Zdenek Kubat, Roman Hobza, Jaroslav Mracek & Boris Vyskot

  2. Laboratory of Molecular Cytogenetics, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 37005, Ceske Budejovice, Czech Republic

    Jiri Macas

Authors
  1. Eduard Kejnovsky
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  2. Zdenek Kubat
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  3. Jiri Macas
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  4. Roman Hobza
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  5. Jaroslav Mracek
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  6. Boris Vyskot
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Corresponding author

Correspondence to Eduard Kejnovsky.

Additional information

Communicated by M.-A. Grandbastien

Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers DQ023669 (Retand-2) and DQ023670 (Retand-1).

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Kejnovsky, E., Kubat, Z., Macas, J. et al. Retand: a novel family of gypsy-like retrotransposons harboring an amplified tandem repeat. Mol Genet Genomics 276, 254–263 (2006). https://doi.org/10.1007/s00438-006-0140-x

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  • DOI: https://doi.org/10.1007/s00438-006-0140-x

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