Abstract
The presence of a monosomic gametocidal chromosome (GC) in a barley chromosome addition line of common wheat generates structural aberrations in the barley chromosome as well as in the wheat chromosomes of gametes lacking the GC. A collection of structurally aberrant barley chromosomes is analogous to a panel of radiation hybrid (RH) mapping and is valuable for high-throughput physical mapping. We developed 90 common wheat lines (GC lines) containing aberrant barley 7H chromosomes induced by a gametocidal chromosome, 2C. DNAs isolated from these GC lines provided a panel of 7H chromosomal fragments in a wheat genetic background, comparable with RH mapping panels in mammals. We used this 7H GC panel and the methodology for RH mapping to physically map PCR-based barley markers, SSRs and AFLPs, onto chromosome 7H, relying on polymorphism between the 7H chromosome and the wheat genome. We call this method GC mapping. This study describes a novel adaptation and combination of methods of inducing chromosomal rearrangements to produce physical maps of markers. The advantages of the presented method are similar to RH mapping in that non-polymorphic markers can be used and the mapping panels can be relatively easily obtained. In addition, mapping results are cumulative when using the same mapping set with new markers. The GC lines will be available from the National Bioresources Project-KOMUGI (http://www.nbrp.jp/index.jsp).
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References
Brandes A, Roder MS, Ganal M (1995) Barley telomeres with two different types of satellite DNA sequences. Chromosome Res 3:315–320
Cox DR, Burmeister M, Price ER, Kim S, Myers RM (1990) Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes. Science 250:245–250
Endo TR (1988) Induction of chromosomal structural changes by a chromosome of Aegilops cylindrica L. in common wheat. J Hered 79:366–370
Endo TR (1990) Gametocidal chromosomes and their induction of chromosome mutations in wheat. Jpn J Genet 65:135–152
Endo TR (2003) Wheat stocks carrying alien chromosomal segments induced by the gametocidal system. In: Proceedings of the 10th international wheat genetics symposium, Paestum, pp 69–72
Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307
Gill KS, Gill BS, Endo TR, Boyko EV (1996) Identification and high-density mapping of gene-rich regions in chromosome group 5 of wheat. Genetics 143(2):1001–1012
Goss SJ, Harris H (1975) New method for mapping genes in human chromosomes. Nature 255:680–684
Janda J, Bartos J, Safar J, Kubalakova M, Valarik M, Cihalikova J, Simkova H, Caboche M, Sourdille P, Bernard M, Chalhoub B, Dolezel J (2004) Construction of a subgenomic BAC library specific for chromosomes 1D, 4D and 6D of hexaploid wheat. Theor Appl Genet 109(7):1337–1345
Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J, Antonio BA, Shomura A, Shimizu T, Lin SY (1994) A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nat Genet 8(4):365–372
Lawrence S, Morton NE, Cox DR (1991) Radiation hybrid mapping. Proc Natl Acad Sci 88:7477–7480
Macaulay M, Ramsay R, Powell P, Waugh R (2001) A representative highly informative ‘genotyping set’ of barley SSRs. Theor Appl Genet 102:801–809
Masoudi-Nejad A, Nasuda S, McIntosh RA, Endo TR (2002) Transfer of rye chromosome segments to wheat by a gametocidal system. Chromosome Res 10:349–357
Matise TC, Perlin M, Chakravarti A (1994) Automated construction of genetic linkage maps using an expert system (MultiMap): a human genome linkage map. Nat Genet 6(4):384–390
Nomura T, Ishihara A, Imaishi H, Ohkawa H, Endo TR, Iwamura H (2003) Rearrangement of the genes for the biosynthesis of benzoxazinones in the evolution of Triticeae species. Planta 217(5):776–782
Qi L, Echalier B, Friebe B, Gill BS (2003) Molecular characterization of a set of wheat deletion stocks for use in chromosome bin mapping of ESTs. Funct Integr Genomics 3(1–2):39–55
Ramsay L, Macaulay M, Ivanissevich S, MacLean K, Cardle L, Fuller J, Edwards KJ, Tuvesson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005
Riera-Lizarazu O, Vales MI, Ananiev EV, Rines HW, Phillips RL (2001) Production characterization of maize chromosome 9 radiation hybrids derived from an oat–maize addition line. Genetics 156:327–339
Safar J, Bartos J, Janda J, Bellec A, Kubalakova M, Valarik M, Pateyron S, Weiserova J, Tuskova R, Cihalikova J, Vrana J, Simkova H, Faivre-Rampant P, Sourdille P, Caboche M, Bernard M, Dolezel J, Chalhoub B (2004) Dissecting large and complex genomes: flow sorting and BAC cloning of individual chromosomes from bread wheat. Plant J 39(6):960–968
Saghai-Maroof MA, Soilman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Serizawa N, Nasuda S, Shi F, Endo TR, Prodanovic S, Schubert I, Künzel G (2001) Deletion-based physical mapping of barley chromosome. Theor Appl Genet 103:827–834
Shi F, Endo TR (1997) Production of wheat-barley disomic addition lines possessing an Aegilops cylindrica gametocidal chromosome. Genes Genet Syst 72:243–248
Shi F, Endo TR (1999) Genetic induction of structural changes in barley chromosomes added to common wheat by a gametocidal chromosome derived from Aegilops cylindrica. Genes Genet Syst 74:49–54
Shi F, Endo TR (2000) Genetic induction of chromosomal rearrangements in barley chromosome 7H added to common wheat. Chromosoma 109(5):358–363
Tsujimoto H, Yamada T, Hasegawa K, Usami N, Kojima T, Endo TR, Ogihara Y, Sasakuma T (2001) Large-scale selection of lines with deletions in chromosome 1B in wheat and applications for fine deletion mapping. Genome 44(4):501–508
Wardrop J, Snape J, Powell W, Machray GC (2002) Constructing plant radiation hybrid panels. Plant J 31(2):223–228
Wardrop J, Fuller J, Powell W, Machray GC, (2004) Exploiting plant somatic radiation hybrids for physical mapping of expressed sequence tags. Theor Appl Genet 108(2):343–348
Acknowledgements
This study was supported in part by a Grant-in-Aid for Scientific Research (A) (No. 123066001) from the Ministry of Education, Science, Sports, and Culture, Japan and facilitated by a BBRSC Japan Partnering award to R. Waugh and T.R. Endo. Technical help from Malcolm Macaulay is much appreciated. Contribution No. 582 from the Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Japan.
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Masoudi-Nejad, A., Nasuda, S., Bihoreau, MT. et al. An alternative to radiation hybrid mapping for large-scale genome analysis in barley. Mol Genet Genomics 274, 589–594 (2005). https://doi.org/10.1007/s00438-005-0052-1
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DOI: https://doi.org/10.1007/s00438-005-0052-1