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Dissection and physical mapping of wheat chromosome 7B by inducing meiotic recombination with its homoeologues in Aegilops speltoides and Thinopyrum elongatum

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Abstract

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We constructed a homoeologous recombination-based bin map of wheat chromosome 7B, providing a unique physical framework for further study of chromosome 7B and its homoeologues in wheat and its relatives.

Abstract

Homoeologous recombination leads to the dissection and diversification of the wheat genome. Advances in genome sequencing and genotyping have dramatically improved the efficacy and throughput of homoeologous recombination-based genome studies and alien introgression in wheat and its relatives. In this study, we aimed to physically dissect and map wheat chromosome 7B by inducing meiotic recombination of chromosome 7B with its homoeologues 7E in Thinopyrum elongatum and 7S in Aegilops speltoides. The special genotypes, which were double monosomic for chromosomes 7B’ + 7E’ or 7B’ + 7S’ and homozygous for the ph1b mutant, were produced to enhance 7B − 7E and 7B − 7S recombination. Chromosome-specific DNA markers were developed and used to pre-screen the large recombination populations for 7B − 7E and 7B − 7S recombinants. The DNA marker-mediated preselections were verified by fluorescent genomic in situ hybridization (GISH). In total, 29 7B − 7E and 61 7B − 7S recombinants and multiple chromosome aberrations were recovered and delineated by GISH and the wheat 90 K SNP assay. Integrated GISH and SNP analysis of the recombinants physically mapped the recombination breakpoints and partitioned wheat chromosome 7B into 44 bins with 523 SNPs assigned within. A composite bin map was constructed for chromosome 7B, showing the bin size and physical distribution of SNPs. This provides a unique physical framework for further study of chromosome 7B and its homoeologues. In addition, the 7B − 7E and 7B − 7S recombinants extend the genetic variability of wheat chromosome 7B and represent useful germplasm for wheat breeding. Thereby, this genomics-enabled chromosome engineering approach facilitates wheat genome study and enriches the gene pool of wheat improvement.

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Acknowledgements

We thank Mary Osenga for her help in high-throughput SNP genotyping and Drs. Lili Qi and Rebekah Oliver for their critical review of the manuscript. This project has been supported by Agriculture and Food Research Initiative Competitive Grant No. 2013-67013-21121 and 2019-67013-29164 from the USDA National Institute of Food and Agriculture.

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Authors and Affiliations

  1. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    Mingyi Zhang, Wei Zhang, Xianwen Zhu & Xiwen Cai

  2. Department of Computer Science, North Dakota State University, Fargo, ND, 58108, USA

    Qing Sun & Changhui Yan

  3. USDA-ARS, Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Center, Fargo, ND, 58102, USA

    Steven S. Xu & Jason Fiedler

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  1. Mingyi Zhang
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  2. Wei Zhang
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Contributions

M.Z. contributed to recombinant production and analysis, SNP assays, GISH, molecular marker development and analysis, and chromosome mapping, and was involved in data analysis and manuscript preparation. W.Z. and X.Z. participated in crossing, chromosome-specific marker analysis, and GISH. Q.S. and C.Y. analyzed DNA sequence data. J.F. ran high-throughput SNP assays. S.S.X. was involved in manuscript preparation. X.C. designed and coordinated this work, and was involved in crosses, data analysis and interpretation, and manuscript preparation.

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Correspondence to Xiwen Cai.

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Zhang, M., Zhang, W., Zhu, X. et al. Dissection and physical mapping of wheat chromosome 7B by inducing meiotic recombination with its homoeologues in Aegilops speltoides and Thinopyrum elongatum. Theor Appl Genet 133, 3455–3467 (2020). https://doi.org/10.1007/s00122-020-03680-3

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