Abstract
In the present study, the second nuclear internal transcribed spacer (ITS-2) rDNA of Schistosoma japonicum isolates in mainland China was amplified, sequenced, and assessed for inferring the intra- and inter-species phylogenetic relationships of trematodes in the order Strigeata. The fragment containing ITS-2 rDNA was obtained from 24 S. japonicum isolates from eight epidemic provinces in mainland China. The length polymorphisms were observed among these ITS-2 rDNA sequences, ranging from 343 to 346 bp, and the intra- and inter-population variations in ITS-2 sequence were 0.0–2.1 % among S. japonicum isolates in China. Phylogenetic analyses using the maximum parsimony and maximum likelihood methods revealed that the ITS-2 rDNA sequence is not a suitable marker for studying inter- and intra-population variation in S. japonicum. However, phylogenetic analysis of trematodes in the order Strigeata indicated that the ITS-2 rDNA sequence provides an effective molecular marker for studying inter-species phylogenetic relationships among trematodes in this order.
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Acknowledgments
Project support was provided, in part, by the National Natural Science Foundation of China (grant no. 30960280), The Program for Outstanding Scientists in Agricultural Research, National Basic Research Program (973 Program) of China (grant no. 2007CB513104), the Open Funds of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (grant nos. SKLVEB2011KFKT011, SKLVEB2010KFKT009, SKLVEB2009KFKT008, SKLVEB2011KFKT010, and SKLVEB2011KFKT004), the Special Funds for Talents in Northwest A & F University (Z109021107 and 2010BSJJ015), and the Yunnan Provincial Program for Introducing High-level Scientists (grant no. 2009CI125). The experiments comply with the current laws of the country in which the experiments were performed.
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Zhao, G.H., Li, J., Mo, X.H. et al. The second transcribed spacer rDNA sequence: an effective genetic marker for inter-species phylogenetic analysis of trematodes in the order Strigeata. Parasitol Res 111, 1467–1472 (2012). https://doi.org/10.1007/s00436-012-2981-z
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DOI: https://doi.org/10.1007/s00436-012-2981-z