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Orientobilharzia turkestanicum is grouped within African schistosomes based on phylogenetic analyses using sequences of mitochondrial genes

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Abstract

In the present study, samples representing Orientobilharzia turkestanicum from cattle, sheep, and cashmere goat in Daqing District, Heilongjiang Province, China, were characterized and grouped genetically by sequences of mitochondrial cytochrome c oxidase subunit 1 gene (cox1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 gene (nad1). Genomic DNAs were extracted from parasites isolated from individual host by sodium dodecyl sulfate-protease K treatment. The cox1 and nad1 genes were amplified by polymerase chain reaction and then sequenced and compared with that of other members of the Schistosomatidae available in GenBankTM, and phylogenetic relationships between them were re-constructed using the neighbor-joining method. The results showed that the lengths of cox1 and nad1 sequences were 1,125 and 518 bp, respectively, for all O. turkestanicum samples sequenced. The identities of cox1 sequences of O. turkestanicum from cattle, sheep, and cashmere goat in Daqing, China with that of O. turkestanicum from sheep in Iran were 99.4%, 99.7%, and 99.8%, respectively. The identities of nad1 sequences of O. turkestanicum from cattle, sheep, and cashmere goat in Daqing, China with that of O. cheni from cashmere goat at Fuyu, Heilongjiang Province, China were 99.4%, 99.0%, and 100%, respectively. Phylogenetic analyses based on the cox1 and nad1 sequences revealed that O. turkestanicum is placed within the African schistosomes, and O. turkestanicum should be considered the sister species of Schistosoma spp.

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Acknowledgments

Project support was provided in part by grants from the Scientific Research Fund of Heilongjiang Provincial Education Department (Grant No. 11511253) to CRW, and from the National Basic Research Program of China (Grant No. 2007CB513104) to XQZ. The experiments comply with the current laws of the country in which the experiments were performed.

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Authors and Affiliations

  1. College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang Province, 163319, People’s Republic of China

    L. Li, C. R. Wang & Y. Q. Zhai

  2. College of Life Science and Technology, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang Province, 163319, People’s Republic of China

    L. Y. Yu

  3. College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, Guangdong Province, 510642, People’s Republic of China

    X. Q. Zhu

  4. Qingdao Lvman Bio-Engineering Co., Ltd, No.360, Qingdao Section of National highway No.308, Licang District, Qingdao, Shandong Province, 266100, People’s Republic of China

    J. P. Zhao

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  1. L. Li
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  2. L. Y. Yu
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  3. X. Q. Zhu
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  4. C. R. Wang
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Correspondence to C. R. Wang.

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Li, L., Yu, L.Y., Zhu, X.Q. et al. Orientobilharzia turkestanicum is grouped within African schistosomes based on phylogenetic analyses using sequences of mitochondrial genes. Parasitol Res 102, 939–943 (2008). https://doi.org/10.1007/s00436-007-0857-4

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  • DOI: https://doi.org/10.1007/s00436-007-0857-4

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