Abstract
The present study studied the genetic variation among Schistosoma japonicum isolates from different endemic regions in mainland China and examined the phylogenetic relationships of zoonotic trematodes using the combined mitochondrial 16S and 12S ribosomal DNA sequences. The fragments of 16S and 12S rDNA were amplified from 22 S. japonicum isolates, and sequenced, and the relevant sequences of other nine trematode species belonging to six genera in four families were downloaded from GenBank, and their phylogenetic relationships were re-constructed by unweighted pair-group method with arithmetic averages analyses using the combined 16S and 12S rDNA sequences, with Trichinella spiralis as outgroup. The results showed that the partial sequences of mitochondrial 16S and 12S rDNA of S. japonicum were 757 and 797 bp, respectively, and they were quite conserved among the S. japonicum isolates. Phylogenetic analysis revealed that the combined 16S and 12S rDNA sequences were not able to distinguish S. japonicum isolates in mountainous areas from those in lake/marshland areas in mainland China. However, the combined sequences could distinguish different species of zoonotic trematodes. Therefore, the combined mitochondrial 16S and 12S rDNA sequences provide an effective molecular marker for the inter-species phylogenetic analysis and differential identification of zoonotic trematodes.
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Acknowledgements
This work is supported, in part, by the National Basic Research Program (973 program) of China (Grant No. 2007CB513104), the Yunnan Provincial Program for Introducing High-level Scientists (Grant No. 2009CI125), the Program for Changjiang Scholars and Innovative Research Team in University (Grant No. IRT0723) to XQZ, and the National Natural Science Foundation of China (Grant No. 30960280) to FCZ. Professor Baozhen Qian of Bioengineering Institute, Zhejiang Academy of Medical Sciences was thanked for providing some S. japonicum samples used in the present study. The experiments comply with the current laws of the country in which the experiments were performed.
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Multiple sequence alignments of combined mitochondrial 12S and 16S rDNA (DOC 132 kb)
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Li, J., Zhao, G.H., Zou, F.C. et al. Combined mitochondrial 16S and 12S rDNA sequences: an effective genetic marker for inter-species phylogenetic analysis of zoonotic trematodes. Parasitol Res 107, 561–569 (2010). https://doi.org/10.1007/s00436-010-1895-x
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DOI: https://doi.org/10.1007/s00436-010-1895-x