Abstract
The beef tapeworm Taenia saginata infects human beings with symptoms ranging from nausea, abdominal discomfort to digestive disturbances and intestinal blockage. In the present study, microRNA (miRNA) expressing profile in adult T. saginata was analyzed using Solexa deep sequencing and bioinformatics analysis. A total of 15.8 million reads was obtained by Solexa sequencing, and 13.3 million clean reads (1.73 million unique sequences) was obtained after removing reads smaller than 18 nt. Ten conserved miRNAs corresponding to 607,382 reads were found when matching the reads against known miRNAs of Schistosoma japonicum in miRBase database. The miR-71 had the most abundant expression in T. saginata, followed by miR-219-5p, but some other common miRNAs such as let-7, miR-40, and miR-103 were not identified in T. saginata. Nucleotide bias analysis found that the known miRNAs showed high bias and the uracil was the dominant nucleotide, particularly at the first and 11th positions which were almost at the beginning and middle of conserved miRNAs. One novel miRNA (Tsa-miR-001) corresponding to ten precursors was identified and confirmed by stem-loop RT-PCR. To our knowledge, this is the first report of miRNA profiles in T. saginata, which will contribute to better understanding of the complex biology of this zoonotic trematode. The reported data of T. saginata miRNAs should provide valuable references for miRNA studies of closed related zoonotic Taenia cestodes such as Taenia solium and Taenia asiatica.
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References
Al-Quraishy S, Delic D, Sies H, Wunderlich F, Abdel-Baki AA, Dkhil MA (2011) Differential miRNA expression in the mouse jejunum during garlic treatment of Eimeria papillata infections. Parasitol Res 109:387–394
Bentwich I, Avniel A, Karov Y, Aharonov R, Gilad S, Barad O, Barzilai A, Einat P, Einav U, Meiri E, Sharon E, Spector Y, Bentwich Z (2005) Identification of hundreds of conserved and nonconserved human microRNAs. Nat Genet 37:766–770
Chen MX, Ai L, Xu MJ, Zhang RL, Chen SH, Zhang YN, Guo J, Cai YC, Tian LG, Zhang LL, Zhu XQ, Chen JX (2011a) Angiostrongylus cantonensis: Identification and characterization of microRNAs in male and female adults. Exp Parasitol 128:116–120
Chen MX, Ai L, Xu MJ, Chen SH, Zhang YN, Guo J, Cai YC, Tian LG, Zhang LL, Zhu XQ, Chen JX (2011b) Identification and characterization of microRNAs in Trichinella spiralis by comparison with Brugia malayi and Caenorhabditis elegans. Parasitol Res 109:553–558
Delić D, Dkhil M, Al-Quraishy S, Wunderlich F (2011) Hepatic miRNA expression reprogrammed by Plasmodium chabaudi malaria. Parasitol Res 108:1111–1121
Du T, Zamore PD (2007) Beginning to understand microRNA function. Cell Res 17:661–663
Hussain M, Taft RJ, Asgari S (2008) An insect virus-encoded microRNA regulates viral replication. J Virol 82:9164–9170
Jeon HK, Eom KS (2006) Taenia asiatica and Taenia saginata: genetic divergence estimated from their mitochondrial genomes. Exp Parasitol 113:58–61
Kaufman EJ, Miska EA (2010) The microRNAs of Caenorhabditis elegans. Semin Cell Dev Biol 21:728–737
Lau NC, Lim LP, Weinstein EG, Bartel DP (2001) An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science 294:858–862
Lees W, Nightingale J, Brown D, Scandrett B, Gajadhar A (2002) Outbreak of Cysticercus bovis (Taenia saginata) in feedlot cattle in Alberta. Can Vet J 43:227–228
Liu Q, Tuo W, Gao H, Zhu XQ (2010) MicroRNAs of parasites: current status and future perspectives. Parasitol Res 107:501–507
Matuchansky C, Lenormand Y (1999) Images in clinical medicine. Taenia saginata. N Engl J Med 341:1737
Mcfadden AM, Heath DD, Morley CM, Dorny P (2011) Investigation of an outbreak of Taenia saginata cysts (Cysticercus bovis) in dairy cattle from two farms. Vet Parasitol 176:177–184
Ogunremi O, Benjamin J (2010) Development and field evaluation of a new serological test for Taenia saginata cysticercosis. Vet Parasitol 169:93–101
Rathjen T, Nicol C, Mcconkey G, Dalmay T (2006) Analysis of short RNAs in the malaria parasite and its red blood cell host. FEBS Lett 580:5185–5188
Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, Horvitz HR, Ruvkun G (2000) The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 403:901–906
Rhoades MW, Reinhart BJ, Lim LP, Burge CB, Bartel B, Bartel DP (2002) Prediction of plant microRNA targets. Cell 110:513–520
Vicentini-Oliveira JC, Golim MA, De Cassia PS, Biondi GF, Rossi-Ferreira R, Deffune E, Nunes CM (2010) Taenia saginata: production and characterization of monoclonal antibodies against Taenia saginata metacestode antigens. Exp Parasitol 126:621–625
Wang Z, Xue X, Sun J, Luo R, Xu X, Jiang Y, Zhang Q, Pan W (2010) An “in-depth” description of the small non-coding RNA population of Schistosoma japonicum schistosomulum. PLoS Negl Trop Dis 4:e596
Wanzala W, Onyango-Abuje JA, Kang’ethe EK, Zessin KH, Kyule NM, Baumann MP, Ochanda H, Harrison LJ (2003) Control of Taenia saginata by post-mortem examination of carcasses. Afr Health Sci 3:68–76
Xu MJ, Liu Q, Nisbet AJ, Cai XQ, Yan C, Lin RQ, Yuan ZG, Song HQ, He XH, Zhu XQ (2010) Identification and characterization of microRNAs in Clonorchis sinensis of human health significance. BMC Genomics 11:521
Xue LJ, Zhang JJ, Xue HW (2009) Characterization and expression profiles of miRNAs in rice seeds. Nucleic Acids Res 37:916–930
Zeiner GM, Norman KL, Thomson JM, Hammond SM, Boothroyd JC (2010) Toxoplasma gondii infection specifically increases the levels of key host microRNAs. PLoS One 5:e8742
Zhang B, Wang Q, Pan X (2007) MicroRNAs and their regulatory roles in animals and plants. J Cell Physiol 210:279–289
Zhang B, Stellwag EJ, Pan X (2009) Large-scale genome analysis reveals unique features of microRNAs. Gene 443:100–109
Acknowledgments
This work is supported in part by the Program for the International Development Research Centre, Canada (Project No. 105509-00001002), National S & T Major Program (grant nos. 2012ZX10004220, 2008ZX10004-011, 2009ZX10004-302 and 2009ZX10004-201), National Key Technology R & D Program (Grant No. 2008BAI56B03), and the Open Funds of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (grant nos. SKLVEB2009KFKT008, SKLVEB2010KFKT009, SKLVEB2011KFKT004, and SKLVEB2011KFKT010). The experiments comply with the current laws of the country in which the experiments were performed.
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L. Ai and M. J. Xu contributed equally to this work.
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Ai, L., Xu, M.J., Chen, M.X. et al. Characterization of microRNAs in Taenia saginata of zoonotic significance by Solexa deep sequencing and bioinformatics analysis. Parasitol Res 110, 2373–2378 (2012). https://doi.org/10.1007/s00436-011-2773-x
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DOI: https://doi.org/10.1007/s00436-011-2773-x