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Molecular and cytogenetic evidence of three sibling species of the Anopheles barbirostris Form A (Diptera:Culicidae) in Thailand

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Abstract

Nine isoline colonies of Anopheles barbirostris Form A, derived from individual isofemale lines from Chiang Mai, Phetchaburi, and Kanchanaburi, were established in our insectary at Chiang Mai University. All isolines shared the same mitotic karyotype (X1, X2, Y1). Molecular analysis of deoxyribonucleic acid (DNA) sequences and polymerase chain reaction (PCR) products of ITS2, COI, and COII regions revealed three distinct groups: A1 (Chiang Mai), A2 (Phetchaburi), and A3 (Kanchanaburi). Crossing experiments among the three groups exhibited strong reproductive isolation, producing low and/or non-hatched eggs, and inviable and/or abnormal development of the reproductive system of F1-progenies. Asynaptic regions along the five polytene chromosome arms of F1-hybrid larvae clearly supported the existence of three sibling species within A. barbirostris Form A, provisionally named species A1, A2, and A3.

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References

  • Baimai V (1977) Chromosomal polymorphisms of constitutive heterochromatin and inversions in Drosophila. Genetics 85:85–93

    Google Scholar 

  • Baimai V, Andre RG, Harrison BA, Kijchalao U, Panthusiri L (1987) Crossing and chromosomal evidence for two additional sibling species within the taxon Anopheles dirus Peyton and Harrison (Diptera: Culicidae) in Thailand. Proc Entomol Soc Wash 89:157–166

    Google Scholar 

  • Baimai V, Rattanarithikul R, Kijchalao U (1995) Metaphase karyotypes of Anopheles of Thailand and Southeast Asia: IV. The barbirostris and umbrosus species groups, subgenus Anopheles (Diptera: Culicidae). J Am Mosq Control Assoc 11:323–328

    Google Scholar 

  • Beebe NW, Saul A (1995) Discrimination of all members of the Anopheles punctulatus complex by polymerase chain reaction-restriction fragment length polymorphism analysis. Am J Trop Med Hyg 53:478–481

    PubMed  CAS  Google Scholar 

  • Chabpunnarat S (1988) Cytogenetic study of the Anopheles maculatus complex. M.Sc. Thesis, Bangkok, Mahidol University

  • Choochote W, Sucharit S, Abeywickreme W (1983) Experiments in crossing two strains of Anopheles barbirostris Van der Wulp 1884 (Diptera: Culicidae) in Thailand. Southeast Asian J Trop Med Public Health 14:204–209

    PubMed  CAS  Google Scholar 

  • Choochote W, Jitpakdi A, Rongsriyam Y, Komalamisra N, Pitasawat B, Palakul K (1998) Isoenzyme study and hybridization of two forms of Anopheles sinensis (Diptera: Culicidae) in northern Thailand. Southeast Asian J Trop Med Public Health 29:841–848

    PubMed  CAS  Google Scholar 

  • Choochote W, Pitasawat B, Jitpakdi A, Rattanachanpichai E, Riyong D, Leemingsawat S, Wongkamchai S (2001) The application of ethanol-extracted Gloriosa superba for metaphase chromosome preparation in mosquitoes. Southeast Asian J Trop Med Public Health 32:76–82

    PubMed  CAS  Google Scholar 

  • Choochote W, Rongsriyam Y, Leemingsawat S, Jitpakdi A, Komalamisara N, Surathin K, Somboon P, Chen B, Wongkamchai S, Jariyapan N, Tippawangkosol P, Pitasawat B, Riyong D (2002) Intraspecific hybridization of Anopheles minimus (Diptera: Culicidae) species A and C in Thailand. Southeast Asian J Trop Med Public Health 33:23–28

    PubMed  Google Scholar 

  • Chowdaiah BN, Avirachan TT, Seetharam PL (1970) Chromosome Studies of Oriental Anophelines. 1. The salivary gland chromosome of Anopheles barbirostris. Experientia 26:315

    Article  PubMed  CAS  Google Scholar 

  • Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3:294–299

    PubMed  CAS  Google Scholar 

  • Harbach RE (2004) The classification of genus Anopheles (Diptera: Culicidae): a working hypothesis of phylogenetic relationships. Bull Entomol Res 94:537–553

    Article  PubMed  CAS  Google Scholar 

  • Harrison BA (1980) Medical entomology studies: XIII. The Myzomyia series of Anopheles (Cellia) in Thailand, with emphasis on intra-interspecific variations (Diptera: Culicidae). Contrib Am Entomol Inst 17:1–195

    Google Scholar 

  • Junkum A, Komalamisra N, Jitpakdi A, Jariyapan N, Min GS, Park MH, Cho KH, Somboon P, Bates PA, Choochote W (2005) Evidence to support two conspecific cytological races of Anopheles aconitus in Thailand. J Vector Ecol 30:213–224

    PubMed  Google Scholar 

  • Kanda T (1979) Improved techniques for the preparation of polytene chromosomes for some Anopheles mosquitoes. Mosq News 39:568–574

    Google Scholar 

  • Kanda T, Takai K, Chiang GL, Cheong WH, Sucharit S (1981) Hybridization and some biological facts of seven strains of the Anopheles leucosphyrus group (Reid, 1968). Jpn J Sanit Zool 32:321–329

    Google Scholar 

  • Kim SJ, Choochote W, Jitpakdi A, Junkum A, Park SJ, Min GS (2003) Establishment of a self-mating mosquito colony of Anopheles sinensis from Korea. Korean Journal of Entomology 33:267–271

    Article  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Tamura K, Nei M (2004) MEGA 3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  PubMed  CAS  Google Scholar 

  • Limrat D, Rojruthai B, Apiwathnasorn C, Samung Y, Prommongkol S (2001) Anopheles barbirostris/campestris as a probable vector of malaria in Aranyaprathet, Sa Kaeo province. Southeast Asian J Trop Med Public Health 32:739–744

    PubMed  CAS  Google Scholar 

  • Min GS, Choochote W, Jitpakdi A, Kim SJ, Jung J, Junkum A (2002) Intraspecific hybridization of Anopheles sinensis (Diptera: Culicidae) strains from Thailand and Korea. Mol Cells 14:198–204

    PubMed  CAS  Google Scholar 

  • Park SJ, Choochote W, Jitpakdi A, Junkum A, Kim SJ, Jariyapan N, Park JW, Min GS (2003) Evidence for a conspecific relationship between two morphologically and cytologically different Forms of Korean Anopheles pullus mosquito. Mol Cells 14:354–360

    CAS  Google Scholar 

  • Rattanarithikul R, Harrison BA, Harbach RE, Panthusiri P, Coleman RE (2006) Illustrated keys to the mosquitoes of Thailand IV. Anopheles. Southeast Asian J Trop Med Public Health 37(Suppl 2):1–128

    Google Scholar 

  • Reid JA (1968) Anopheline mosquitoes of Malaya and Borneo. Stud Inst Med Res Malaya 31:1–520

    Google Scholar 

  • Saeung A, Otsuka Y, Baimai V, Somboon P, Pitasawat B, Tuetun B, Junkum A, Takaoka H, Choochote W (2007) Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand. Parasitol Res 101:1337–1344

    Article  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Sallum MAM, Peyton EL, Wilkerson RC (2005) Six new species of the Anopheles leucosphyrus group, reinterpretation of An. elegans and vector implications. Med Vet Entomol 19:158–199

    Article  PubMed  CAS  Google Scholar 

  • Sawadipanich Y, Baimai V, Harrison BA (1990) Anopheles dirus species E: chromosomal and crossing evidence for another member of the dirus complex. J Am Mosq Control Assoc 6:477–481

    PubMed  CAS  Google Scholar 

  • Scanlon JE, Peyton EL, Gould DJ (1968) An annotated checklist of the Anopheles of Thailand. Thai National Science Papers, Fauna Series 2:1–35

    Google Scholar 

  • Sharpe RG, Harbach RE, Butlin RK (2000) Molecular variation and phylogeny of members of the Minimus group of Anopheles subgenus Cellia (Diptera: Culicidae). Syst Entomol 25:263–272

    Article  Google Scholar 

  • Somboon P, Walton C, Sharpe RG, Higa Y, Tuno N, Tsuda Y, Takagi M (2001) Evidence for a new sibling species of Anopheles minimus from the Ryukyu Archipelago, Japan. J Am Mosq Control Assoc 17:98–113

    PubMed  CAS  Google Scholar 

  • Somboon P, Thongwat D, Choochote W, Walton C, Takagi M (2005) Crossing experiments of Anopheles minimus species C and putative species E. J Am Mosq Control Assoc 21:5–9

    Article  PubMed  Google Scholar 

  • Takai K, Kanda T, Ogawa KI, Sucharit S (1987) Morphological differentiation in Anopheles maculatus of Thailand accompanied with genetical divergence assessed by hybridization. J Am Mosq Control Assoc 3:148–153

    PubMed  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors sincerely thank the Biodiversity Research and Training Program (Grant No. BRT_249004), the Thailand Research Fund through the Royal Golden Jubilee Ph.D Program (Grant No. PHD/0052/2548) and the Faculty of Medicine Endowment Fund for financially supporting this research project, and Dr. Niwes Nantachit, Dean of the Faculty of Medicine, Chiang Mai University for his interest in this research project.

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand

    Atiporn Saeung, Pradya Somboon, Anuluck Junkum, Benjawan Tuetun & Wej Choochote

  2. Department of Biology and Center for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand

    Visut Baimai

  3. Department of Infectious Disease Control, Faculty of Medicine, Oita University, Hasama, Oita, 879-5593, Japan

    Yasushi Otsuka & Hiroyuki Takaoka

  4. Department of Entomology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand

    Rampa Rattanarithikul

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  1. Atiporn Saeung
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  2. Visut Baimai
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  3. Yasushi Otsuka
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  4. Rampa Rattanarithikul
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  5. Pradya Somboon
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  6. Anuluck Junkum
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  8. Hiroyuki Takaoka
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  9. Wej Choochote
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Correspondence to Wej Choochote.

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Saeung, A., Baimai, V., Otsuka, Y. et al. Molecular and cytogenetic evidence of three sibling species of the Anopheles barbirostris Form A (Diptera:Culicidae) in Thailand. Parasitol Res 102, 499–507 (2008). https://doi.org/10.1007/s00436-007-0788-0

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  • DOI: https://doi.org/10.1007/s00436-007-0788-0

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