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Cloning and characterization of the mitochondrial heat-shock protein 60 gene of Trichinella spiralis

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Abstract

The full-length cDNA of mitochondrial heat-shock protein (hsp) 60 of the infective-stage larva of Trichinella spiralis was cloned by degenerative PCR and rapid amplification of cDNA end reactions. The 1,945 bp full-length cDNA sequence contained an open reading frame of 576 amino acids. A mitochondrial signal peptide was located at the N-terminal and a GGM motif at the C-terminal. The gene contained ten exons and nine introns. RT-PCR analysis indicated that thermal, cold, acidic and oxidative treatment did not elicit significant changes in the expression of mitochondrial hsp 60 in the larvae. Cluster analysis showed that the sequence of the hsp 60 gene of T. spiralis is closely related to that of Drosophila melanogaster.

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Acknowledgements

This study was supported by a grant from the Hong Kong Research Council (HKU 426/96M) to R.C.K. and the Conference and Research Grants of the University of Hong Kong to R.C.K. and C.H.M. We are grateful to the invaluable assistance of Ms. Y.Y.Y. Chung.

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Authors and Affiliations

  1. 5S-13, Kadoorie Biological Sciences Building, Department of Zoology, The University of Hong Kong, Hong Kong SAR, P.R.China

    C. S. Wong, C. H. Mak & R. C. Ko

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  1. C. S. Wong
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  2. C. H. Mak
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  3. R. C. Ko
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Correspondence to R. C. Ko.

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Wong, C.S., Mak, C.H. & Ko, R.C. Cloning and characterization of the mitochondrial heat-shock protein 60 gene of Trichinella spiralis. Parasitol Res 93, 461–467 (2004). https://doi.org/10.1007/s00436-004-1156-y

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  • DOI: https://doi.org/10.1007/s00436-004-1156-y

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