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Genome-wide identification and expression analysis of the cotton patatin-related phospholipase A genes and response to stress tolerance

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Abstract

Main conclusion

Patatin-related phospholipase A genes were involved in the response of Gossypium hirsutum to drought and salt tolerance.

Abstract

pPLA (patatin-related phospholipase A) is a key enzyme that catalyzes the initial step of lipid hydrolysis, which is involved in biological processes, such as drought, salt stress, and freezing injury. However, a comprehensive analysis of the pPLA gene family in cotton, especially the role of pPLA in the response to drought and salt tolerance, has not been reported so far. A total of 33 pPLA genes were identified in this study using a genome-wide search approach, and phylogenetic analysis classified these genes into three groups. These genes are unevenly distributed on the 26 chromosomes of cotton, and most of them contain a few introns. The results of the collinear analysis showed that G. hirsutum contained 1–5 copies of each pPLA gene found in G. arboreum and G. raimondii. The subcellular localization analysis of Gh_D08G061200 showed that the protein was localized in the nucleus. In addition, analysis of published upland cotton transcriptome data revealed that six GhPLA genes were expressed in various tissues and organs. Two genes (Gh_A04G142100.1 and Gh_D04G181000.1) were highly expressed in all tissues under normal conditions, showing the expression characteristics of housekeeping genes. Under different drought and salt tolerance stresses, we detected four genes with different expression levels. This study helps to clarify the role of pPLA in the response to drought and salt tolerance.

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Data sharing does not apply to this article as no datasets were generated during the current study.

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Funding

This study was supported by the National Natural Science Foundation of China (5201101621). This work was performed at the China–Pakistan Joint Laboratory for Cotton Biotechnology, Beijing, China.

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Author notes

  1. Yunxiao Wei, Zhili Chong, and Chao Lu have contributed equally to this work.

Authors and Affiliations

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing, 100081, China

    Yunxiao Wei, Zhili Chong, Chao Lu, Kaili Li, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo & Rui Zhang

  2. College of Plant Science, Tarim University, 1487 East Tarim Avenue, Aral City, 843300, China

    Zhili Chong & Liangrong He

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  1. Yunxiao Wei
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Wei, Y., Chong, Z., Lu, C. et al. Genome-wide identification and expression analysis of the cotton patatin-related phospholipase A genes and response to stress tolerance. Planta 257, 49 (2023). https://doi.org/10.1007/s00425-023-04081-8

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