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Genome-wide analysis of the bZIP gene lineage in apple and functional analysis of MhABF in Malus halliana

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Abstract

Main Conclusion

51 MdbZIP genes were identified from the apple genome by bioinformatics methods. MhABF-OE improved tolerance to saline–alkali stress in Arabidopsis, indicating it is involved in positive regulation of saline–alkali stress response.

Abstract

Saline–alkali stress is a major abiotic stress limiting plant growth all over the world. Members of the bZIP family play an important role in regulating gene expression in response to many kinds of biotic and abiotic stress, including salt stress. According to the transcriptome data, 51 MdbZIP genes responding to saline–alkali stress were identified in apple genome, and their gene structures, conserved protein motifs, phylogenetic analysis, chromosome localization, and promoter cis-acting elements were analyzed. Based on transcriptome data analysis, a MdbZIP family gene (MD15G1081800), which was highly expressed under stress, was selected to isolate and named as MhABF. Expression profile analysis by quantitative real-time PCR confirmed that the expression of MhABF in the leaves of Malus halliana was 10.6-fold higher than that of the control (0 days) after 2 days of stress. Then an MhABF gene was isolated from apple rootstock M. halliana. CaMV35S promoter drived MhABF gene expression vector was constructed to infect Arabidopsis with Agrobacterium-mediated infection. And overexpression MhABF gene plants were obtained. Compared with wild type, transgenic plants grew better under saline–alkali stress and the MhABF-OE lines showed higher chlorophyll content, POD, SOD and CAT activity, which indicated that they had strong resistance to stress. These results indicate that MhABF plays an important role in plant resistance to saline–alkali stress, which lays a foundation for further study on the functions in apple.

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Acknowledgements

This work was supported by Special Fund for Discipline Construction of Gansu Agricultural University (GSAU-XKJS-2018-221) and National Natural Science Foundation of China (Project Number 31960581).

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Authors and Affiliations

  1. College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, People’s Republic of China

    Shuangcheng Wang, Rui Zhang, Zhongxing Zhang, Ting Zhao, De Zhang, Yuxia Wu & Yanxiu Wang

  2. Institute of Agriculture and Environment, Massey University, Palmerston North, 4442, New Zealand

    Svetla Sofkova

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  1. Shuangcheng Wang
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Wang, S., Zhang, R., Zhang, Z. et al. Genome-wide analysis of the bZIP gene lineage in apple and functional analysis of MhABF in Malus halliana. Planta 254, 78 (2021). https://doi.org/10.1007/s00425-021-03724-y

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