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Molecular cloning and characterization of OsCHR4, a rice chromatin-remodeling factor required for early chloroplast development in adaxial mesophyll

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Abstract

Mi-2 protein, the central component of the NuRD nucleosome remodeling and histone deacetylase complex, plays a role in transcriptional repression in animals. Mi-2-like genes have been reported in Arabidopsis, though their function in monocots remains largely unknown. In the present study, a rice Mi-2-like gene, OsCHR4 (Oryza sativa Chromatin Remodeling 4, LOC_Os07g03450), was cloned from a rice mutant with adaxial albino leaves. The Oschr4 mutant exhibited defective chloroplasts in adaxial mesophyll, but not in abaxial mesophyll. Ultrastructural observations indicated that proplastid growth and/or thylakoid membrane formation in adaxial mesophyll cells was blocked in the Oschr4 mutant. Subcellular localization revealed that OsCHR4::GFP fusion protein was targeted to the nuclei. OsCHR4 was mainly expressed in the root meristem, flower, vascular bundle, and mesophyll cells by promoter::GUS analysis in transgenic rice. The transcripts of some nuclear- and plastid-encoded genes required for early chloroplast development and photosynthesis were decreased in the adaxial albino mesophyll of the Oschr4 mutant. These observations provide evidence that OsCHR4, the rice Mi-2-like protein, plays an important role in early chloroplast development in adaxial mesophyll cells. The results increase our understanding of the molecular mechanism underlying tissue-specific chloroplast development in plants.

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Abbreviations

CC:

Chloroplast-containing

CHD:

Chromodomain helicase DNA-binding

EMS:

Ethyl methanesulfonate

GFP:

Green fluorescent protein

LCM:

Laser capture microdissection

Mi-2/NuRD:

Multi-subunit chromatin remodeling complex

NCC:

No chloroplast-containing

NEP:

Nuclear encoded RNA polymerase

ORF:

Open reading frame

OsCHR4:

Oryza sativa chromatin remodeling 4

PEP:

Plastid encoded RNA polymerase

qRT-PCR:

Quantitative real-time PCR

RT-PCR:

Reverse transcription PCR

STS:

Sequence tagged site

TEM:

Transmission electron microscopy

WT:

Wild type

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Acknowledgments

This work was financially supported by the National Basic Research and Development Program of China (2011CB100300), the Ministry of Agriculture of China (2011ZX08009-003-005) and the National Natural Science Foundation (30971703, 30971742).

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Authors and Affiliations

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Chunfang Zhao, Jiming Xu, Yue Chen, Chuanzao Mao, Shelong Zhang, Youhuang Bai, Dean Jiang & Ping Wu

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  1. Chunfang Zhao
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  2. Jiming Xu
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  3. Yue Chen
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  4. Chuanzao Mao
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Correspondence to Ping Wu.

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Zhao, C., Xu, J., Chen, Y. et al. Molecular cloning and characterization of OsCHR4, a rice chromatin-remodeling factor required for early chloroplast development in adaxial mesophyll. Planta 236, 1165–1176 (2012). https://doi.org/10.1007/s00425-012-1667-1

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