Abstract
A 1887-nucleotide chloroplast-DNA region from Arabidopsis thaliana was analyzed. It contains the conserved genes psbA for the precursor of the D1 reaction-centre protein of photosystem II, trnH for tRNAHis, and rps19′ for the 6.8-kDa protein of the small ribosomal subunit. Northern hybridization and RNase protection experiments suggest co-transcription of a minor RNA fraction over the full lengths of psbA and the preceding trnK-UUU gene, but not including downstream trnH sequences. In front of the mapped 5′ end of the major 1.2-kb psbA transcript is a DNA region that shows the typical architecture of a psbA promoter, consisting of the prokaryotic-type ‘-35’ and ‘-10’ elements as well as the eukaryotic-type ‘TATA’ motif. The common 3′ end of psbA transcripts seems to be located immediately after a stem-loop structure downstream from the coding region.
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Aldrich J, Cherney BW, Merlin E, Christopherson L (1988) The role of insertions/deletions in the evolution of the intergenic region between psbA and trnH in the chloroplast genome. Curr Genet 14:137–146
Christopher DA, Mullet JE (1994) Separate photosensory pathways co-regulate blue light/ultraviolet-A-activated psbD-psbC transcription and light-induced D2 and CP43 degradation in barley (Hordeum vulgare) chloroplasts. Plant Physiol 104:1119–1129
Eisermann A, Tiller K, Link G (1990) In vitro transcription and DNA-binding characteristics of chloroplast and etioplast extracts from mustard (Sinapis alba) indicate differential usage of the psbA promoter. EMBO J 9:3981–3987
Gruissem W, Zurawski G (1985) Analysis of promoter regions of the spinach chloroplast rbcl, atpB, and psbA genes. EMBO J 4:3375–3383
Hughes JE, Neuhaus H, Link G (1987) Transcript levels of two adjacent chloroplast genes during mustard (Sinapis alba L.) seedling development are under differential temporal and light control. Plant Mol Biol 9:355–363
Lidholm J, Gustafsson P (1992) A functional promoter shift of a chloroplast gene: a transcriptional fusion between a novel psbA gene copy and the trnK(uuu) gene in Pinus contorta. Plant J 2:875–886
Link G (1984) DNA sequence requirements for the accurate transcription of a protein-coding plastid gene in a plastid in vitro system from mustard (Sinapis alba L.). EMBO J 3:1697–1704
Link G, Langridge U (1984) Structure of the chloroplast gene for the precursor of the Mr 32,000 photosystem II protein from mustard (Sinapis alba L.). Nucleic Acids Res 12:945–958
Link G (1994) Plastid differentiation: organelle promoters and transcription factors. In: Nover L (ed) Plant promoters and transcription factors. Springer, Berlin Heidelberg New York, pp 65–85
Meyerowitz EM (1989) Arabidopsis, a useful weed. Cell 56:263–269
Nickelsen J, Link G (1991) RNA-protein interactions at transcript 3′ ends and evidence for trnK-psbA co-transcription in mustard chloroplasts. Mol Gen Genet 228:89–96
Nickelsen J, Link G (1993) The 54-kDa RNA-binding protein from mustard chloroplasts mediates endonucleolytic transcript 3′-end formation in vitro. Plant J 3:537–544
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Stevenson JK, Hallik RB (1994) The psbA operon pre-mRNA of the Euglena gracilis chloroplast is processed into photosystem I and II mRNAs that accumulate differentially depending on the conditions of cell growth. Plant J 5:247–260
Sugita M, Sugiura M (1984) Nucleotide sequence and transcription of the gene for the 32 000-dalton thylakoid membrane protein from Nicotiana tabacum. Mol Gen Genet 195:308–313
Sugiura M (1992) The chloroplast genome. Plant Mol Biol 19:149–168
Zurawski G, Bottomley W, Whitfeld PR (1984) Junctions of the large single-copy region and the inverted repeats in Spinacea oleracea and Nicotiana debneyi chloroplast DNA: sequence of the genes for tRNA His and the ribosomal proteins S19 and L2. Nucleic Acids Res 12:6547–6557
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Communicated by H. Kössel
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Liere, K., Kestermann, M., Müller, U. et al. Identification and characterization of the Arabidopsis thaliana chloroplast DNA region containing the genes psbA, trnH and rps19′ . Curr Genet 28, 128–130 (1995). https://doi.org/10.1007/BF00315778
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DOI: https://doi.org/10.1007/BF00315778