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SWI2/SNF2 chromatin remodeling ATPases SPLAYED and BRAHMA control embryo development in rice

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Chromatin remodeling ATPases OsSYD and OsBRM are involved in shoot establishment, and both affect OSH gene transcription. OsSYD protein interacts with RFL, but OsBRM does not.

Abstract

In plants, SPLAYED (SYD) and BRAHMA (BRM) encode chromatin remodeling ATPases that use the energy derived from ATP hydrolysis to restructure nucleosomes and render certain genomic regions available to transcription factors. However, the function of SYD and BRM on rice growth and development is unknown. Here, we constructed ossyd and osbrm mutants using CRISPR/Cas9 technology and analyzed the effects of mutations on rice embryo development. We discovered that the ossyd and osbrm mutants exhibited severe defects during embryonic development, whereas endosperm development was normal. These results indicated that the development of the embryo and endosperm is independent of each other. Consequently, the ossyd- and osbrm-null mutants did not germinate due to the abnormal embryos. Furthermore, we observed the embryos of ossyd- and osbrm-null mutants, and they indeed had distinct differentiation defects in shoot establishment, acquired during embryogenesis. To verify the function of OsSYD and OsBRM in embryogenesis, we measured the transcript levels of marker genes at different stages. Compared with wild type, the expression levels of multiple OSH genes were significantly reduced in the mutants, which was consistent with the defective shoot establishment phenotypes. The interaction between SYD and RICE FLORICAULA/LFY (RFL) was revealed using a yeast two-hybrid screening system, suggesting that the interaction between the LFY homolog and chromatin remodeling ATPases is ubiquitous in plants. Collectively, our findings provide the basis for elucidating the function of OsSYD and OsBRM during embryo development in rice.

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Acknowledgements

The authors thank Pro. Jiankang Zhu and Pro. Caixia Gao for offering the CRISPR/Cas9 gene-editing vector, and thank Pro. Junko Kyozuka for the gift of rfl/apo2 mutant seeds. We also appreciate the Biogle and Biorun Geneme Editing Center for producing transgenic rice.

Funding

This study was supported by the National Natural Science Foundation of China (Grant number 31872855 and 31971842).

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Authors and Affiliations

  1. College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Qian Xun, Min Mei, Ye Song, Chenyu Rong, Jiajun Liu, Tianhui Zhong, Yanfeng Ding & Chengqiang Ding

  2. Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of Agriculture, Nanjing, 210095, People’s Republic of China

    Yanfeng Ding & Chengqiang Ding

  3. Collaborative Innovation Center for Modern Crop Production Co-Sponsored by Province and Ministry, Nanjing, 210095, People’s Republic of China

    Yanfeng Ding & Chengqiang Ding

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  1. Qian Xun
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  2. Min Mei
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  5. Jiajun Liu
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Contributions

QX, CD and YD designed the research. QX, MM, YS, CR, JL, TZ and CD performed the experiments. QX and CD analyzed the data and prepared the paper.

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Correspondence to Chengqiang Ding.

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The authors declare no conflict of interest.

Accession numbers

Gene information in this article can be found in Rice Annotation Project database, miRbase data libraries and the Arabidopsis Information Resource are under the corresponding accession numbers: OsUBQ (Os03g0234200), OsSYD (Os06g0255200), RFL/APO2 (Os04g0598300), OsBRM (Os02g0114000), Roc1 (Os08g0187500), OsSCR (Os12g0122000), OsPNH1 (Os06g0597400), OSH1 (Os03g0727000), OSH6 (Os01g0302500), OSH15 (Os07g0129700), OSH71(Os05g0129700), RAmy1A(Os02g0765600).

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Communicated by Xian Sheng Zhang.

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Xun, Q., Mei, M., Song, Y. et al. SWI2/SNF2 chromatin remodeling ATPases SPLAYED and BRAHMA control embryo development in rice. Plant Cell Rep 41, 1389–1401 (2022). https://doi.org/10.1007/s00299-022-02864-z

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