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Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor

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Abstract

Annexins are multifunctional proteins characterized by their capacity to bind calcium ions and negatively charged lipids. Although there is increasing evidence implicating their importance in plant stress responses, their functions in seeds remain to be further studied. In this study, we identified a heat-induced annexin, NnANN1, from the embryonic axes of sacred lotus (Nelumbo nucifera Gaertn.) using comparative proteomics approach. Moreover, the expression of NnANN1 increased considerably in response to high-temperature treatment. Quantitative real-time PCR (qRT-PCR) revealed that the transcripts of NnANN1 were detected predominantly during seed development and germination in sacred lotus, implicating a role for NnANN1 in plant seeds. Ectopic expression of NnANN1 in Arabidopsis resulted in enhanced tolerance to heat stress in transgenic seeds. In addition, compared to the wild-type seeds, transgenic seeds ectopically expressing NnANN1 exhibited improved resistance to accelerated aging treatment used for assessing seed vigor. Furthermore, transgenic seeds showed enhanced peroxidase activities, accompanied with reduced lipid peroxidation and reduced ROS release levels compared to the wild-type seeds. Taken together, these results indicate that NnANN1 plays an important role in seed thermotolerance and germination vigor.

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Abbreviations

AA:

Accelerated aging

ANN:

Annexin

CHAPS:

3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate

Cys:

Cysteine

DCFH-DA:

2′,7′-Dichlorofluorescein-diacetate

DTT:

Dithiothreitol

ECL:

Enhanced chemiluminescence

EDTA:

Ethylenediaminetetraacetic acid

H2O2 :

Hydrogen peroxide

IPTG:

Isopropyl-β-thiogaloctopyranoside

MALDI-TOF MS:

Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry

PMSF:

Phenylmethylsulfonyl fluoride

RH:

Relative humidity

ROS:

Reactive oxygen species

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Tyr:

Tyrosine

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Acknowledgments

This work was supported by the Guangdong Agriculture Science and Technology Team Project (2011A02010210), the Natural Science Foundation of China (No. 30370912, 30971564 and 90919038) and Natural Science Foundation of Guangdong Province (2006B20101010 and 9151027501000075).

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Plant Resource, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China

    Pu Chu, Huhui Chen, Yuliang Zhou, Yin Li & Shangzhi Huang

  2. School of Life Sciences, Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

    Yu Ding & Liwen Jiang

  3. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada

    Edward W. T. Tsang

  4. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Keqiang Wu

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  1. Pu Chu
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Chu, P., Chen, H., Zhou, Y. et al. Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor. Planta 235, 1271–1288 (2012). https://doi.org/10.1007/s00425-011-1573-y

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