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Nodulin 22 from Phaseolus vulgaris protects Escherichia coli cells from oxidative stress

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Abstract

Plant genes that are induced during the formation and function of a root nodule are called nodulin genes. Cloning and functional analysis of nodule-specific gene products are of valuable help in establishing the role and requirements of the host plant for the specificity and effectiveness of the symbiosis. A cDNA clone (nod22) was isolated from Phaseolus vulgaris L. (common bean) cDNA library derived from Rhizobium -infected roots. Nodulin 22 (Nod22) transcripts are accumulated from early to late stages in root nodule development. RT-PCR in situ studies indicated that Nod22 transcripts are highly accumulated in cortical, vascular bundle and infected cells. The deduced Nod22 protein contains a highly hydrophobic N-terminus, with signal peptide characteristics, and a C-terminal extension with high identity to the α-crystallin domains found in α-crystallin lens chaperone, and other small heat-shock proteins. These domains have not been previously described in other known nodulins, but have been observed in small heat-shock proteins found in plant tissues exposed to elevated temperature and oxidative stress. Nod22, when it is over-expressed in Escherichia coli, cells confers protection against oxidative stress suggesting its possible role in plant host protection from oxidative toxicity during the Rhizobium-legume symbiosis.

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Abbreviations

ACD :

α-Crystallin domain

CLSM :

Confocal laser scanning microscopy

sHsp :

Small heat-shock protein

SP :

Signal peptide

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Acknowledgements

Assistance by Noreide Nava and Gabriel Guillén is appreciated. We gratefully acknowledge Xóchitl Alvarado for the confocal laser scanning microscopy, and Nayeli Sánchez for processing the images in Adobe Photoshop. Dr Otto Gieger’s critical review of the manuscript is appreciated. This research was funded by projects DGAPA (IN232002), and CONACYT (33350-N).

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Authors and Affiliations

  1. Departamento de Biología Molecular de Plantas, Universidad Nacional Autónoma de México, Apartado Postal 510–3, 62271, Cuernavaca, Morelos, México

    Asif Mohammad, Georgina Estrada Navarrete, Carmen Quinto, Juan Elías Olivares, Berenice García-Ponce & Federico Sánchez

  2. Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510–3, 62271, Cuernavaca, Morelos, México

    Juan Miranda-Ríos

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  1. Asif Mohammad
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  2. Juan Miranda-Ríos
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  7. Federico Sánchez
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Correspondence to Federico Sánchez.

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Mohammad, A., Miranda-Ríos, J., Estrada Navarrete, G. et al. Nodulin 22 from Phaseolus vulgaris protects Escherichia coli cells from oxidative stress. Planta 219, 993–1002 (2004). https://doi.org/10.1007/s00425-004-1303-9

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  • DOI: https://doi.org/10.1007/s00425-004-1303-9

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