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Proteomic analysis of embryo development in rice (Oryza sativa)

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Abstract

Although embryo development is a major subject in plant growth and development research, a number of aspects of the mechanism of this development process remain unknown. Rice (Oryza sativa) is an excellent monocot plant model for studying embryogenesis with a known genome sequence. Here, we conducted proteomic analysis of embryo development in rice (O. sativa L. ssp. indica cv. 9311). The aim was to investigate and characterize the changes in the protein expression profile during embryo development. For this purpose, the proteome of developing embryos was characterized by two-dimensional gel electrophoresis and nano liquid chromatography/mass spectrometry/mass spectrometry. Proteomic analyses identified 275 differentially expressed proteins throughout the 5 sequential developmental stages from 5 to 30 days after pollination. Most of these proteins were classified into eight functional categories: metabolism, protein synthesis/destination, disease and defense, transporter, transcription, signal transduction, cell growth/division, and storage proteins, which were involved in different cellular and metabolic processes. Hierarchical clustering analyses of protein expression profiles showed that highly expressed proteins in early stages were involved in metabolism, protein synthesis/destination, and most of the other cellular functions, whereas the proteins highly expressed in later stages functioned in the desiccation and dormancy of the embryo.

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Abbreviations

DAP:

Days after pollination

FBA:

Fructose-bisphospate aldolase

GAPDH:

Glyceraldehyde 3-P dehydrogenase

PGAM:

2, 3-bisphosphoglycerate-independent phosphoglycerate mutase

LEA:

Late embryogenesis abundant

PFK:

Pyrophosphate-dependent phosphofructokinase

PFP:

Pyrophosphate-fructose 6-phosphate 1-phosphotransferase

TCA:

Tricarboxylic acid

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Acknowledgments

We thank Dr. Tai Wang (Institute of Botany, Chinese Academy of Sciences, China) for his help in 2-DE technique. This work was supported by the State Key Basic Research and Development Plan of China (2007CB108700), and the National Natural Science Foundation of China (30821064).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of MOE for Plant Development Biology, State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China

    Hong Xu, Weiping Zhang, Yi Gao, Yong Zhao, Lin Guo & Jianbo Wang

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  1. Hong Xu
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  2. Weiping Zhang
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  3. Yi Gao
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  4. Yong Zhao
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Correspondence to Jianbo Wang.

Electronic supplementary material

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425_2011_1535_MOESM1_ESM.xls

Supplementary Table S1 Data obtained by nano-LC-MS/MS and protein database search for 242 differentially expressed protein spots. Sequence information obtained from nano-LC/MS/MS of a single excised protein spot was taken and submitted to MASCOT (Matrix Science Ltd.) search engine for protein identification using the Institute of Genomic Research (TIGR) protein database (XLS 314 kb)

425_2011_1535_MOESM2_ESM.doc

Supplementary Table S2 A catalog of identified proteins differentially expressed during embryo development in rice (DOC 599 kb)

425_2011_1535_MOESM3_ESM.xls

Supplementary Table S3 The proteins distribution in hierarchical clusters and the expression level of the differentially expressed proteins within each cluster. The following hierarchical clusters were created by GeneCluster 2.0. The proteins of unknown function were not listed (XLS 67 kb)

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Xu, H., Zhang, W., Gao, Y. et al. Proteomic analysis of embryo development in rice (Oryza sativa). Planta 235, 687–701 (2012). https://doi.org/10.1007/s00425-011-1535-4

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