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Peroxisomal localisation of the final steps of the mevalonic acid pathway in planta

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Abstract

In plants, the mevalonic acid (MVA) pathway provides precursors for the formation of triterpenes, sesquiterpenes, phytosterols and primary metabolites important for cell integrity. Here, we have cloned the cDNA encoding enzymes catalysing the final three steps of the MVA pathway from Madagascar periwinkle (Catharanthus roseus), mevalonate kinase (MVK), 5-phosphomevalonate kinase (PMK) and mevalonate 5-diphosphate decarboxylase (MVD). These cDNA were shown to functionally complement MVA pathway deletion mutants in the yeast Saccharomyces cerevisiae. Transient transformations of C. roseus cells with yellow fluorescent protein (YFP)-fused constructs reveal that PMK and MVD are localised to the peroxisomes, while MVK was cytosolic. These compartmentalisation results were confirmed using the Arabidopsis thaliana MVK, PMK and MVD sequences fused to YFP. Based on these observations and the arguments raised here we conclude that the final steps of the plant MVA pathway are localised to the peroxisome.

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Abbreviations

AACT:

Acetoacetyl-CoA thiolase

FOA:

5-fluoroorotic acid

HMGR:

3-hydroxy-3-methylglutaryl-CoA reductase

HMGS:

3-hydroxy-3-methylglutaryl-CoA synthase

IDI:

Isopentenyl diphosphate isomerase

MVA:

Mevalonic acid

MVD:

Mevalonate 5-diphosphate decarboxylase

MVK:

Mevalonate kinase

PMK:

5-phosphomevalonate kinase

PTS:

Peroxisomal targeting signal

YFP:

Yellow fluorescent protein

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Acknowledgments

We thank “Le STUDIUM” (Agency for Research and Hosting Foreign associated Researchers in the Centre region, France) for the financial support of Andrew J. Simkin. Grégory Guirimand is the recipient of a Ph.D. fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche (France). Insaf Thabet is supported by a cotutelle Ph.D. fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie).

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Authors and Affiliations

  1. EA 2106, Biomolécules et Biotechnologies Végétales, Université François-Rabelais de Tours, 31 Avenue Monge, 37200, Tours, France

    Andrew J. Simkin, Grégory Guirimand, Nicolas Papon, Vincent Courdavault, Insaf Thabet, Olivia Ginis, Nathalie Giglioli-Guivarc’h & Marc Clastre

  2. Laboratoire de Biotechnologie et Physiologie Végétale, Département des Sciences Biologiques, Faculté des Sciences de Tunis, El-Manar II, 2092, Tunis, Tunisia

    Insaf Thabet & Sadok Bouzid

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  1. Andrew J. Simkin
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Correspondence to Andrew J. Simkin.

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Simkin, A.J., Guirimand, G., Papon, N. et al. Peroxisomal localisation of the final steps of the mevalonic acid pathway in planta . Planta 234, 903–914 (2011). https://doi.org/10.1007/s00425-011-1444-6

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