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Over-expression of osa-MIR396c decreases salt and alkali stress tolerance

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Abstract

Salt and alkali stress are two of the main environmental factors limiting rice production. Thus, understanding the mechanisms of salinity and alkali stress tolerance is necessary to modify rice to increase its resistance to salinity and alkaline stress. MicroRNAs (miRNAs) are ~21-nucleotide RNAs that are ubiquitous regulators of gene expression in eukaryotic organisms. In plants, miRNAs constitute one of five classes of small RNAs that function primarily as negative regulators for gene expression at the posttranscriptional level. Several plant miRNAs, such as miR396, play vital roles in plant growth, development and resistance to stresses. In this study, we identified osa-MIR396c, which shows dramatic transcript change under salt and alkali stress conditions in Oryza sativa. We designed an experiment to detect miRNA–target interaction and demonstrated that several transcription factors related to growth, development, and stress tolerance are targeted by osa-MIR396c. Transgenic rice and Arabidopsis thaliana plants constitutively over-expressing osa-MIR396c showed reduced salt and alkali stress tolerance compared to that of wild-type plants. Overall, this study further established a link between salt and alkali stress and osa-MIR396c in rice.

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Abbreviations

miRNA:

MicroRNA

TF:

Transcription factor

GRF:

Growth-regulating factor

MS-medium:

Murashige and Skoog medium

IRRI:

International Rice Research Institute

GEO:

Gene expression omnibus

NCBI:

National Center for Biotechnology Information

PCR:

Polymerase chain reaction

RT-PCR:

Reverse transcription PCR

pre-miRNA:

miRNA precursor

RGRC:

Rice Genome Resource Center

GFP:

Green fluorescent protein

PGR:

Plant growth regulator

ABA:

Abscisic acid

RACE:

Rapid amplification of cDNA ends

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Acknowledgments

This project was supported by a grant from the Key Research Plan of Heilongjiang Province (GA06B103-3), the Innovation Research Group of NEAU (CXT004), the “863” project (2008AA10Z153), and the Basic Research Preliminary Study Foundation of the Ministry of Science and Technology of the PRC (2003CCA03500).

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Authors and Affiliations

  1. Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin, 150030, China

    Peng Gao, Xi Bai, Liang Yang, Dekang Lv, Yong Li, Hua Cai, Wei Ji & Yanming Zhu

  2. State Key Lab of Agrobiotechnology, Department of Biology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong

    Dianjing Guo

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  1. Peng Gao
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  2. Xi Bai
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  3. Liang Yang
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  4. Dekang Lv
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  5. Yong Li
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  6. Hua Cai
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  7. Wei Ji
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  8. Dianjing Guo
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  9. Yanming Zhu
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Correspondence to Yanming Zhu.

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Gao, P., Bai, X., Yang, L. et al. Over-expression of osa-MIR396c decreases salt and alkali stress tolerance. Planta 231, 991–1001 (2010). https://doi.org/10.1007/s00425-010-1104-2

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  • DOI: https://doi.org/10.1007/s00425-010-1104-2

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