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Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana

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Abstract

Transgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H2O2 production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H2O2 and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H2O2 derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana.

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Abbreviations

[Ca2+]cyto :

Concentration of cytosolic calcium ions

DMTU:

Dimethylthiourea

DHC:

2,5-Dihydroxycinnamic acid

DPI:

Diphenylene iodonium

LaCl3 :

Lanthanum chloride

RR:

Ruthenium red

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Acknowledgments

This work was supported by the grant of National Natural Science Foundation in China (No. 30570157) for Weiming Cai, the grant of National Natural Science Foundation (No. 30871704) and 100 Talents Program of The Chinese Academy of Sciences for Xiangyang Hu. We thank Dr Knight H (University of Oxford, Oxford, UK) kindly provided the pMAQ 2.4 plasmid. We also thank Professor Thorsten Nürnberger (Institute of Plant Biochemistry, Weinberg, Germany) who provided Pep-25, and Professor Gregg A. Howe (Michigan State University, USA) who provided tomato AOS antibody. Finally, we also are grateful to Dr Carl Johnson (Vanderbilt University, Nashville, USA) for critical reading of the manuscript.

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Authors and Affiliations

  1. Kunming Institute of Botany, Institute of Tibet Plateau Research at Kunming, Chinese Academy of Science, Kunming, 650204, China

    Xiangyang Hu & Yongping Yang

  2. Centre for Research in Plant Science, University of the West of England, Bristol, Coldharbour Lane, Bristol, BS16 1QY, UK

    Stevn J. Neill

  3. Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 2000032, China

    Weiming Cai

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  1. Xiangyang Hu
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  2. Stevn J. Neill
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  3. Yongping Yang
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  4. Weiming Cai
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Correspondence to Xiangyang Hu or Weiming Cai.

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Hu, X., Neill, S.J., Yang, Y. et al. Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana . Planta 229, 1201–1208 (2009). https://doi.org/10.1007/s00425-009-0909-3

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