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Establishment of a soybean (Glycine max Merr. L) transposon-based mutagenesis repository

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Abstract

Soybean is a major crop species providing valuable feedstock for food, feed and biofuel. In recent years, considerable progress has been made in developing genomic resources for soybean, including on-going efforts to sequence the genome. These efforts have identified a large number of soybean genes, most with unknown function. Therefore, a major research priority is determining the function of these genes, especially those involved in agronomic performance and seed traits. One means to study gene function is through mutagenesis and the study of the resulting phenotypes. Transposon-tagging has been used successfully in both model and crop plants to support studies of gene function. In this report, we describe efforts to generate a transposon-based mutant collection of soybean. The Ds transposon system was used to create activation-tagging, gene and enhancer trap elements. Currently, the repository houses approximately 900 soybean events, with flanking sequence data derived from 200 of these events. Analysis of the insertions revealed approximately 70% disrupted known genes, with the majority matching sequences derived from either Glycine max or Medicago truncatula sequences. Among the mutants generated, one resulted in male-sterility and was shown to disrupt the strictosidine synthase gene. This example clearly demonstrates that it is possible to disrupt soybean gene function by insertional mutagenesis and to derive useful mutants by this approach in spite of the tetraploid nature of the soybean genome.

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Acknowledgments

This work was supported by grants provided through USDA/NRI Award No. 2002-35301-12195, and the United Soybean Board. Additional funds provided through the National Center for Soybean Biotechnology and Nebraska Research Initiative.

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Authors and Affiliations

  1. National Center for Soybean Biotechnology, Division of Plant Sciences, Life Sciences Center, University of Missouri, Rollins Road, Columbia, MO, 65211, USA

    Melanie Mathieu, Jinrong Wan, Shaoxing Wang, Zhanyuan Zhang, Henry Nguyen & Gary Stacey

  2. Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA

    Elizabeth K. Winters, Fanming Kong & Tom Clemente

  3. Department of Agronomy, Iowa State University, G405 Agronomy Hall, Ames, IA, 50011, USA

    Helene Eckert, Diane Luth, Margie Paz & Kan Wang

  4. USDA-ARS-CICGRU, Iowa State University, G405 Agronomy Hall, Ames, IA, 50011, USA

    Randy C. Shoemaker

  5. Division of Biochemistry, Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO, 65211, USA

    Gary Stacey

  6. Center for Biotechnology, Center for Plant Science Innovation, University of Nebraska, Lincoln, NE, 68588, USA

    Tom Clemente

  7. Department of Agronomy and Plant Genetics, University of Minnesota, Saint-Paul, MN, 55108, USA

    Christopher Donovan & David Somers

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  1. Melanie Mathieu
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  2. Elizabeth K. Winters
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  3. Fanming Kong
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  4. Jinrong Wan
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  5. Shaoxing Wang
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  7. Diane Luth
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  9. Christopher Donovan
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  10. Zhanyuan Zhang
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  11. David Somers
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  12. Kan Wang
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  13. Henry Nguyen
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  14. Randy C. Shoemaker
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  16. Tom Clemente
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Correspondence to Tom Clemente.

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Mathieu, M., Winters, E.K., Kong, F. et al. Establishment of a soybean (Glycine max Merr. L) transposon-based mutagenesis repository. Planta 229, 279–289 (2009). https://doi.org/10.1007/s00425-008-0827-9

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  • DOI: https://doi.org/10.1007/s00425-008-0827-9

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